DNA VACCINATION FOR PREVENTION OF VIRAL, PARASITIC AND MYCOBACTERIAL INFECTION

用于预防病毒、寄生虫和分枝杆菌感染的 DNA 疫苗接种

• 批准号: 6288990
• 负责人: ROBERT A SEDER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6288990
• 关键词: Leishmania major; Mycobacterium tuberculosis; Orthomyxoviridae; active immunization; antibody formation; cytokine; laboratory mouse; protein biosynthesis; vaccine development; vector vaccine

Purpose-Immunization with plasmid DNA has been shown to induce protective immunity in a variety of experimental models of infection. The ability of DNA vaccination to elicit both MHC class I and class II restricted T cell responses has been shown to be responsible for mediating these protective immune responses. Thus, DNA vaccination can provide a useful and an effective way in providing effective immunity to particular pathogens depending on the type of immunity required for protection. We have identified a number of murine experimental models in which to develop an understanding for the utility of DNA vaccination. For infectious diseases, this includes murine models for Leishmania major (L. major), Mycobacterium tuberculosis and Influenza. In addition this work is being applied to primate studies using DNA for HIV-specific antigens. The work involves identifying cloned antigens for the specific infectious agent and cloning it into an appropriate euykaryotic expression vector. In addition, we have obtained DNA for a number of cytokines and costimulatory molecules to be used as adjuvants to the specific antigens. Following purification of the DNA, mice are then injected and boosted several weeks later. At various times following the vaccination, mice are infected with a particular pathogen. Parameters that are followed include survival, quantitation of infectious burden, and immunologic studies of antibody and cytokine production. - Leishmania, HIV, Tuberculosis, Cellular Immunity, Cytokines, Vaccines, CD8+ T Cells, Memory

用质粒DNA的目的免疫已显示出在多种实验模型的感染模型中诱导保护性免疫。 DNA疫苗接种引起MHC I类和II类限制的T细胞反应的能力已被证明是介导这些保护性免疫反应的原因。因此，根据保护所需的免疫力类型，DNA疫苗接种可以为对特定病原体提供有效免疫的有效和有效的方法。我们已经确定了许多鼠实验模型，在这些模型中，可以对DNA疫苗接种的实用性产生理解。对于传染病，这包括利什曼原虫大调的鼠模型（L. Major），结核分枝杆菌和流感。此外，这项工作将用于使用HIV特异性抗原DNA的灵长类动物研究。这项工作涉及鉴定特定感染剂的克隆抗原，并将其克隆到适当的欧亚卡二氧载体中。此外，我们已经获得了许多细胞因子和共刺激分子的DNA，可用作特定抗原的佐剂。纯化DNA后，将小鼠注射并在几周后增强。在接种疫苗后的不同时间，小鼠被特定的病原体感染。遵循的参数包括存活，传染负担的定量以及抗体和细胞因子产生的免疫学研究。 -Leishmania，HIV，结核病，细胞免疫，细胞因子，疫苗，CD8+ T细胞，记忆