SPINDLE GEOMETRY & METAPHASE ANAPHASE CHECKPOINT CONTROL

主轴几何形状

• 批准号: 6280696
• 负责人: GREENFIELD SLUDER
• 金额: $ 0.27万
• 依托单位: WADSWORTH CENTER
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-01-01 至 1998-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6280696
• 关键词: animal tissue; biomedical resource; growth /development; microscopy; urinary tract

(Supported in part by NIH BM 30758 to G. Sluder and NIH R01 GM40198 to C. Rieder). It has been hypothesized that animal cells delay anaphase onset, and thus exit from mitosis, when the mitotic spindle lacks a normal bipolar symmetry. To evaluate this hypothesis we used the BMIRR video-LM facility to film multinucleated PtK1 cells that formed multipolar spindles. An analysis of the data revealed that anaphase onset is delayed in monopolar spindles containing unattached kinetochores, but not in multipolar spindles which do not contain unattached kinetochores. This data reveals that the metaphase-anaphase Cellular cycle checkpoint does not monitor spindle geometry. It further suggests that monopolar spindles are check pointed not because they lack the proper spindle geometry, but because they have unattached kinetochores that signal wait anaphase. Sluder, G., E.A. Thompson, F.J. Miller, J. Hayes and C.L. Rieder. 1997. The checkpoint control for anaphase onset does not monitor excess numbers of spindle poles or bipolar spindle symmetry. J. Cell Sci., 110:421-429.

（部分由 NIH BM 30758 G. Sluder 和 NIH R01 支持 GM40198 至 C. Rieder）。 据推测，动物细胞 延迟后期开始，从而退出有丝分裂，当有丝分裂 纺锤体缺乏正常的双极对称性。 评估这个假设 我们使用 BMIRR video-LM 设备来拍摄多核 PtK1 细胞 形成多极纺锤体。 对数据的分析显示 单极纺锤体的后期开始被延迟 独立的动粒，但不在多极纺锤体中，多极纺锤体不 含有独立的动粒。 该数据表明 中期-后期 细胞周期检查点不监测纺锤体 几何学。 它进一步表明单极纺锤体受到检查 指出不是因为它们缺乏适当的主轴几何形状，而是因为 它们具有未附着的着丝粒，发出等待后期的信号。 斯鲁德, G、E.A.汤普森、F.J. 米勒、J. 海耶斯和 C.L.里德。 1997。 后期开始的检查点控制不监测过量的数量 纺锤体极或双极纺锤体对称。 细胞科学杂志， 110：421-429。