Flotillin link membrane microdomains to signalling endosome during T cell activation.

在 T 细胞激活过程中，Flotillin 将膜微结构域与信号内体连接起来。

• 批准号: DE140101626
• 负责人: Dr Jeremie Rossy
• 金额: $ 27.56万
• 依托单位: The University of New South Wales
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Early Career Researcher Award
• 财政年份: 2014
• 资助国家: 澳大利亚
• 起止时间: 2014-01-01 至 2017-08-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DE140101626
• 关键词: Flotillin; link; membrane; microdomains; signalling

This project aims to determine the mechanisms that connect signalling microdomains at the cell surface to intracellular signalling endosomes to regulate T cell activation. A T cell immune response begins with the reorganisation of the plasma membrane to yield two-dimensional signalling microdomains that must be connected to the three-dimensional microarchitecture of the endocytic matrix for full T cell activation. This project hypothesises that Flotillin form distinct signalling microdomains in the plasma membrane that internalise to constitute an independent endocytic pathway. Using single-molecule and ultra-fast fluorescence imaging, the project will demonstrate that Flotillin represent a unique two-dimensional to three-dimensional regulatory mechanism for T cell signalling.

该项目旨在确定将细胞表面信号微结构域与细胞内信号内体连接以调节 T 细胞激活的机制。 T 细胞免疫反应始于质膜重组，产生二维信号传导微结构域，必须将其连接到内吞基质的三维微结构上，以实现 T 细胞的完全激活。该项目假设 Flotillin 在质膜中形成独特的信号微结构域，这些微结构域内化形成独立的内吞途径。该项目将利用单分子和超快荧光成像，证明 Flotillin 代表了 T 细胞信号传导的独特二维至三维调节机制。