CORE--TISSUE ACQUISITION AND CELL CULTURE

核心——组织采集和细胞培养

• 批准号: 6110950
• 负责人: WALTER E FINKBEINER
• 金额: $ 18.09万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-01 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6110950
• 关键词: biomedical facility; cystic fibrosis; human tissue; respiratory system; tissue /cell culture

Basic research toward understanding the pathobiology of cystic fibrosis requires a continuing and dependable source of cells, affected and unaffected by the disease. Thus, the primary objective of the Core Cell Culture Facility is to acquire airway tissues and culture airway surface epithelial and tracheobronchial gland cells. A tissue procurement system has been established for obtaining normal and cystic fibrosis airway tissue. Once obtained, surface epithelial tissue is enzymatically digested and liberated cells are established in primary culture. Tracheobronchial gland cells are isolated as acini and expanded through a single passage. Surface epithelial and gland cell cultures are evaluated for differentiated properties using light microscopy, electron microscopy and measurements of short circuit current and transpithelial resistance. Cells showings satisfactory features are distributed to investigators. If surplus cells are available, these are frozen for use later. Additionally, established cell lines routinely used by investigators are maintained in the Core Cell Culture Facility. The final aim of the Cell Culture Core Facility is to continue efforts to improve cultures, particularly those derived from tracheobronchial gland cells and to develop cell culture model systems applicable to the studies proposed by SCOR investigators. With respect to improving in vitro cell differentiation, both soluble factors (growth factors, hormones, chemicals) added to culture medium and insoluble factors (extracellular matrix components, air interface culture) are tested for their effects on airway gland cell growth and differentiation. Culture conditions that allow full expression of ion transport and mucus secretory function are two of the goals of this aim. However, the highest priority will be given to determining the culture conditions that promote serous gland cell differentiation.

了解囊性纤维化病理学的基础研究 需要持续且可靠的细胞来源，受影响和 不受疾病的影响。因此，核心细胞的主要目标 培养设施将获取气道组织和培养气道表面 上皮和气管腺细胞。组织采购系统 已建立用于获得正常和囊性纤维化气道的 组织。一旦获得，表面上皮组织被酶促消化 在原发性培养物中建立了解放的细胞。气管bronchial 将腺细胞分离为acini，并通过单个传导扩展。 评估表面上皮和腺细胞培养物的 使用光学显微镜，电子显微镜和 短回路电流和换皮电阻的测量。细胞 显示令人满意的特征是分发给研究人员的。如果 剩余的细胞可用，这些细胞被冷冻以供以后使用。此外， 调查人员通常使用的已建立细胞系在 核心细胞培养设施。细胞培养核心的最终目标 设施将继续努力改善文化，尤其是那些文化 源自气管腺体细胞并发展细胞培养 模型系统适用于SCOR研究人员提出的研究。 关于改善体外细胞分化，两者都可溶 因素（生长因子，激素，化学物质）添加到培养基和 不溶性因子（细胞外基质组件，空气界面培养） 对其对气道腺细胞生长的影响和 分化。允许完全表达离子的培养条件 运输和粘液分泌功能是该目标的两个目标。 但是，确定文化的最高优先级 促进浆液腺细胞分化的条件。