Imaging and Histology Core

成像和组织学核心

• 批准号: 7688322
• 负责人: C. William Davis
• 金额: $ 18.23万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7688322
• 关键词: Animals; Area; Arts; Biological; Biology; Biomedical Research; Categories; Communities; Complement; Confocal Microscopy; Consultations; Cystic Fibrosis; Data; Development; Discipline; Disease; Dissection; Effectiveness; Electron Microscopy; Electron Microscopy Facility; Exhibits; Freeze Etching; Freeze Fracturing; Freezing; Gamma Cameras; Gene Expression; Gene Transfer; Genomics; Glycocalyx; Hand; Hemophilia A; Histology; Human; Image; Imagery; Imaging technology; Investigational Therapies; Knowledge; Lasers; Life; Light; Lung; Measurement; Methodology; Microscope; Microscopy; Mission; Modality; Molecular; Molecular Medicine; Mucins; Mucous body substance; North Carolina; Organ; Plastics; Procedures; Pulmonary Cystic Fibrosis; Radionuclide Imaging; Research; Research Personnel; Research Project Grants; Resources; Scanning; Series; Services; Software Tools; Specificity; Specimen; Techniques; Technology; Therapeutic Studies; Training; Transmission Electron Microscopy; Universities; Viral; Viral Vector; Virus Diseases; Waxes; Work; adeno-associated viral vector; airway surface liquid; base; charge coupled device camera; digital imaging; epon; falls; gastrointestinal system; gene therapy; human disease; improved; instrumentation; interest; light microscopy; novel; novel therapeutics; programs; technique development; therapeutic transgene; tissue processing; treatment center; vector

The MTCC Imaging & Histology Core offers a suite of modern digital imaging services to biomedical nvestigators at the University of North Carolina at Chapel Hill working to develop gene therapy delivery vectors and procedures. Briefly, the Core will support efforts of gene therapy investigators working to optimize the replication and targeting of adeno-associated viral vectors, and lentiviral and paramyxoviral vectors. Additionally, the Core will also support efforts to understand the functional barrier to viral infection offered by mucus and the luminal glycocalyx in the airways of the lung and in the gastrointestinal system, with the aim of improving delivery of viral vectors through the mucus/mucin barriers of these organs. The support offered by the Core falls into three categories. First, the Core offers small animal and macro imaging services to UNC gene therapy facilities, in the form of cryo-cooled CCD-luminometry and conventional CCD cameras for gene expression studies, gamma-camera scintigraphy for lung clearance studies, and CCD camera-equipped fluorescent stereo dissection microscopy for varied purposes. Second, the Core offers a full range of modern digital imaging microscopy services, with a suite of conventional wide field and laser scanning confocal microscopes to gene therapy investigators. Third, investigators also have available full service histology and electron microscopy facilities. With all these services, investigators also receive consultation with technical problems and assistance with problems in image quantitation and data extraction. The services offered are fully consistent with the importance of modern digital image technology to molecular medicine and the development of effective gene therapies.

MTCC 成像和组织学核心为生物医学提供一套现代数字成像服务 北卡罗来纳大学教堂山分校的研究人员致力于开发基因疗法 载体和程序。简而言之，核心将支持基因治疗研究人员的努力 优化腺相关病毒载体、慢病毒和副粘病毒载体的复制和靶向 向量。此外，核心还将支持了解病毒感染的功能屏障的努力 由肺部气道和胃肠系统中的粘液和管腔糖萼提供， 目的是改善病毒载体通过这些器官的粘液/粘蛋白屏障的传递。这 核心提供的支持分为三类。首先，Core 提供小动物和宏观成像 以低温冷却 CCD 发光测定法和传统 CCD 的形式为 UNC 基因治疗设施提供服务 用于基因表达研究的相机、用于肺清除研究的伽马相机闪烁扫描仪和 CCD 用于各种目的的配备摄像头的荧光立体解剖显微镜。其次，Core 提供了 全方位的现代数字成像显微镜服务，包括一套传统的宽视场和激光 向基因治疗研究人员提供扫描共焦显微镜。第三，调查人员还拥有完整的 服务组织学和电子显微镜设施。通过所有这些服务，调查人员还可以获得 咨询技术问题并协助解决图像定量和数据提取方面的问题。 所提供的服务完全符合现代数字图像技术对分子生物学的重要性。 医学和有效基因疗法的开发。