CORE--CELL CULTURE

核心--细胞培养

• 批准号: 6109988
• 负责人: WALTER E FINKBEINER
• 金额: $ 11.7万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-01 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6109988
• 关键词: biomedical facility; cell differentiation; cystic fibrosis; cytology; electrophysiology; growth media; human subject; northern blottings; nucleic acid probes; oligonucleotides; respiratory epithelium; tissue /cell culture

Basic research toward understanding the pathobiology of cystic fibrosis requires a continuing and dependable source of cells, obtained from patients with and without the disease. Thus, the primary objective of the CORE Airway Cell Culture Facility is to acquire airway tissues and culture airway surface epithelial and tracheobronchial gland cells. A tissue procurement system for obtaining normal and CF airway tissue has already been established. Once obtained, tissue is enzymatically digested and the dispersed cells are established in primary culture. Tracheobronchial gland cells are expanded through a single passage. Surface epithelial and gland cell cultures are evaluated for differentiated properties using light microscopy, electron microscopy and measurements of short circuit current and transepithelial resistance. Confluent cell sheets are distributed to investigators. Additionally, established cell lines routinely used by investigators are maintained and distributed by the CORE Cell Culture Facility. The Cell Culture CORE will also continue its efforts to improve cultures, particularly those derived from tracheobronchial gland cells. The effects of media additives (growth factors, hormones, other chemicals) on airway gland cell growth and differentiation will be tested. The effects of air interface feeding and of a variety of growth supports (e.g. different filters, various collagens and extracellular matrix components) will also be assessed. These experiments should allow us to determine a relatively simple set of culture conditions which allows full expression of ion transport and mucus secretory function from low plating densities.

了解囊性纤维化病理学的基础研究 需要从 有和没有疾病的患者。因此， 核心气道细胞培养设施是获取气道组织和培养物 气道表面上皮和气管腺细胞。组织 获得正常和CF气道组织的采购系统已经 已建立。 一旦获得，组织就会消化，并 分散的细胞在原发性培养中建立。 气管bronchial 腺细胞通过单个通道扩展。 表面上皮和 使用使用腺体细胞培养物评估了使用的分化特性 光学显微镜，电子显微镜和短回路的测量值 电流和旋转电阻。汇合细胞表是 分发给调查人员。另外，已建立的细胞系 调查人员通常使用核心使用和分发 细胞培养设施。细胞培养核心也将继续 改善文化的努力，尤其是从 气管粘腺细胞。媒体添加剂的影响（增长 气道腺细胞生长的因素，激素，其他化学物质） 分化将进行测试。空气界面进料的影响和 各种增长支持（例如不同的过滤器，各种胶原蛋白 还将评估细胞外基质组件）。 这些 实验应允许我们确定一组相对简单的集合 可以完全表达离子运输和粘液的培养条件 低电镀密度的分泌功能。