CORE--CELL MODELS

核心--细胞模型

• 批准号: 7052594
• 负责人: WALTER E FINKBEINER
• 金额: $ 13.62万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7052594
• 关键词: biomedical facility; cell bank /registry; cystic fibrosis; drug discovery /isolation; human tissue; metabolism disorder chemotherapy; respiratory epithelium; small molecule; tissue /cell culture

Basic pharmacological research aimed at identifying drugs that may correct the cellular derangements that lead to the debilitating and often fatal lung disease associated with cystic fibrosis requires a continuing and dependable source of cystic fibrosis and "normal" respiratory tissues. Cell culture techniques that allow the propagation of respiratory epithelial cells and formation of differentiated cell culture model systems provide a necessary and powerful tool for testing candidate drugs. Thus, the primary objective of the Cell Models Core is the collection of CF and non-CF respiratory tract tissues and the preparation of differentiated cultures of the respiratory surface epithelial cells and the tracheobronchial submucosal gland cells. A secondary objective of the Cell Models Core is to identify cell culture conditions that permit full expression of differentiated cellular functions, particularly in cell cultures that have been expanded by serial propagation. A tissue procurement system has been established for obtaining both CF and control nasal and airway tissues. Procured tissue is enzymatically digested, and liberated surface epithelial and submucosal gland acinar cells are established as cell cultures. The surface epithelial cells are studied as primary cell cultures of highly differentiated cell sheets that mimic the structure and function of the native nasal and airway epithelium. To increase the number of cell sheets available for screening compounds, some cell cultures are also expanded by serial propagation. The initial primary cultures of the tracheobronchial gland cells are expanded and then seeded as secondary cultures for experimental use. The conditions in which the passaged gland cells are grown are manipulated to produce cells expressing either a mucous or serous cell phenotype. Having respiratory epithelial cells derived from both the mucosal surface and the submucosal gland cells allows investigators to test candidate drugs for action in the various respiratory tract cells most affected in cystic fibrosis.

基本的药理学研究旨在鉴定可能纠正细胞的药物 导致与囊性相关的衰弱和致命的肺部疾病的危险 纤维化需要持续且可靠的囊性纤维化来源和“正常”呼吸道 组织。细胞培养技术允许呼吸性上皮细胞和 差异化细胞培养模型系统的形成为 测试候选药物。因此，细胞模型核心的主要目的是收集CF和非CF呼吸道组织，以及制备呼吸表面上皮细胞的分化培养物和气管粘膜粘膜粘膜粘膜细胞。细胞模型核心的次要目标是识别允许分化细胞功能完全表达的细胞培养条件，尤其是在通过串行传播扩展的细胞培养物中。已经建立了组织采购系统，用于获得CF和对照鼻组织和气道组织。采购的组织是酶促消化的，解放的表面上皮和粘膜粘膜腺泡细胞被确定为细胞培养。将表面上皮细胞研究为模仿天然鼻和气道上皮的结构和功能的高分分化细胞表的原代细胞培养物。为了增加可用于筛选化合物的细胞表数量，一些细胞培养物也通过串行传播扩展。气管腺细胞的初始培养物扩展，然后作为二级培养物进行实验用途。操纵过传来的腺细胞生长的条件以产生表达粘液或浆液细胞表型的细胞。具有源自粘膜表面和粘膜粘膜细胞的呼吸性上皮细胞 允许研究人员在大多数呼吸道细胞中测试候选药物是否在各种呼吸道细胞中进行作用 受到囊性纤维化的影响。