PRIMATE MODEL OF MENOPAUSAL HOT FLASHES

更年期潮热的灵长类动物模型

• 批准号: 6116465
• 负责人: ROBERT FREEDMAN
• 金额: $ 5.85万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6116465
• 关键词: Mammalia; aging; clinical research; endocrine gland /system; model design /development; technology /technique; women's health

OBJECTIVE To determine whether skin temperature and conductance (an electrical measure of sweating) could be measured in rhesus macaques for development of this animal model in the study of the etiology of hot flashes. RESULTS The animal was not anesthetized. Electrodes and thermisters, for measuring skin conductance and temperature, respectively, were applied to the ear pinna, forehead, calf and chest of a female rhesus macaque positioned in a restraining chair to which the animal had been previously accustomed for prolonged periods. The animal was allowed ad libitum access to food and water, and was watched continuously during data recording which took place in a room under precise temperature and humidity control. Data were recorded on two days for three to five hours each day. On the first day, baseline measurements were obtained for 90 minutes after which the room temperature raised and lowered a few degrees for 30 minutes then held at 35{C for approximately 30 minutes. Skin temperatures were stable during the baseline period and then showed the expected physiological responses to the ambient temperature changes. During the 30 minute heating period, fluctuations in skin conductance level sugges tive of sweating were seen in the calf. On the second day of data recording, the same animal was used. Baseline measurements were obtained for 125 minutes after which the ambient temperature was slowly raised to 40{C. Skin temperatures were stable during baseline, after which they increased with ambient temperature. Skin conductance level suggestive of sweating was seen in the sternum and calf during the heating period. The data demonstrate the feasibility of the proposed recording procedures. FUTURE DIRECTIONS We plan to conduct three short pilot studies in ovariectomized female macaques in the coming year to identify and validate one or more objective physiological markers that would indicate the occurance of a hot flash. KEY WORDS menopause, hot flashes, skin temperature; skin conductance; animal model development. FUNDING NIH PO1 AG11915, R37 AG05233, R21 application pending.

目的确定皮肤温度和电导是否 （出汗的电量度）可以在恒河猴中测量 用于开发这种动物模型的猕猴 潮热的病因。 结果该动物没有麻醉。 电极和热器，用于测量皮肤电导和 温度分别应用于耳朵pinna，额头， 雌性猕猴的小腿和胸部放在限制中 动物以前习惯了延长的椅子 时期。 可以随意进入食物和水， 并在发生的数据录制期间不断观看 在精确温度和湿度控制下的房间。 数据是 每天记录两天，持续三到五个小时。 在第一个 一天，获得基线测量90分钟之后 室温升高并降低了几度30分钟 然后保持在35 {C约30分钟。 皮肤温度 在基线期间稳定，然后显示预期 对环境温度变化的生理反应。 期间 30分钟加热期，皮肤电导水平的波动 在小牛中看到了出汗的建议。 第二天 数据记录，使用了相同的动物。 基线测量是 获得125分钟，然后环境温度为 慢慢提高到40 {c。 在基线期间，皮肤温度稳定， 之后，它们随环境温度的增加。 皮肤电导 在胸骨和小腿中看到了表现出汗的水平 加热期。 数据证明了 拟议的记录程序。 我们计划进行的未来指示 三项在卵巢切除的女猕猴中的三项简短试点研究 来年确定和验证一个或多个目标 生理标志物，表明发生热 闪光。 关键单词更年期，潮热，皮肤温度；皮肤 电导；动物模型发展。 资助NIH PO1 AG11915，R37 AG05233，R21申请待定。