EMBRYO VIABILITY TESTING

胚胎活力测试

• 批准号: 6568409
• 负责人: DAVID KEEFE
• 金额: $ 21.13万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6568409
• 关键词: Mammalia; aging; animal tissue; bioengineering /biomedical engineering; biomedical equipment development; biomedical resource; child (0-11); clinical research; female; growth /development; male; mother /infant health care; reproductive system; technology /technique; women's health

This subproject focuses on a major problem in the health of American women specifically addressing the question of age related oocyte dysfunction and infertility. Our goal is to develop instrumentation capable of determining the viability of oocytes and preimplantation embryos. With this instrument, a new derivative of the self-referencing technology, we are using indicators of metabolic activity or cell physiology, to assay viability. Our work to date has focussed on the mouse oocyte or embryo but we will soon move to consider the hamster oocyte, which shows an age-related dysfunction similar of the human. Our initial studies in this area used the Seris calcium electrode to measure transmembrane ion flux and successfully demonstrated differences between embryos that went on to divide in culture and those that did not. Most recently, however, we have begun to apply the newly developed Serp-oxygen electrodes. The results are very encouraging. Not only can changes in oxygen consump-tion be discriminated after fertilization but also an increase is recorded as the embryo develops to a blastocyst. Most surprisingly we have been able to record oxygen consumption from the oocyte, a feat commonly held to be impossible. The use of this technology has required considerable changes in the normal operating procedure, including the regulation of temperature and careful handling. Most recently the two P.I.s on this project have acquired funding to develop a tailor made system to be housed in the Laboratory for Reproductive Medicine and operated by a full time associate. We expect to see rapid progress in this field over the next year.

该子项目重点关注人类健康的一个重大问题 美国女性专门解决与年龄相关的问题 卵母细胞功能障碍和不孕。 我们的目标是发展 能够确定卵母细胞活力的仪器 植入前胚胎。 有了这个工具，一个新的衍生品 自参考技术，我们使用代谢指标 活性或细胞生理学，以测定活力。 迄今为止我们的工作已经 重点关注小鼠卵母细胞或胚胎，但我们很快就会转向 以仓鼠卵母细胞为例，它显示出与年龄相关的功能障碍 与人类相似。 我们在该领域的初步研究使用了 Seris 钙电极成功测量跨膜离子通量 证明了继续分裂的胚胎之间的差异 文化和那些没有文化的人。 然而，最近我们已经开始 应用新开发的Serp氧电极。 结果是 非常令人鼓舞。 不仅可以改变耗氧量 受精后区分，但也增加记录为 胚胎发育成囊胚。 最令人惊讶的是我们 能够记录卵母细胞的耗氧量，这是一项常见的壮举 被认为是不可能的。 使用该技术需要 正常操作程序发生重大变化，包括 调节温度并小心处理。 最近两人 该项目的 P.I. 已获得资金来开发定制的 系统将安装在生殖医学实验室和 由全职员工操作。 我们预计将在以下方面看到快速进展 明年这个领域。