BIACORE 2000 PROCESSING UNIT

BIACORE 2000 处理单元

基本信息

批准号：
2766833
负责人：
UTTAM L RAJBHANDARY
金额：
$ 23.8万
依托单位：
MASSACHUSETTS INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
1999
资助国家：
美国
起止时间：
1999-03-15 至 2000-03-14
项目状态：
已结题

项目摘要

The application by five P.I.'s proposes to use surface plasmon resonance for the kinetic and thermodynamic analysis of specific protein-protein and protein-nucleic acid interactions in a variety of biological systems. The RAJBHANDARY laboratory will perform studies of protein synthesis initiation in eubacteria and in eukaryotes. The molecular mechanisms underlying the specific interaction between the E. coli and human initiator transfer RNAs and the corresponding protein synthesis initiation factors IF2 and eIF2 will be studied using wild type and mutant initiator transfer RNAs. The KHORANA laboratory will study binding and kinetic constants of protein-protein interactions in visual processes (sensitization and desensitization) following light-activation of the vertebrate photoreceptor rhodopsin. Initial focus will be on interactions between activated receptor and the G protein, transducin, on the one hand and between the receptor and rhodopsin kinase on the other. The KIM laboratory will study peptides from the core of HIV gp41, that are known to be inhibitors of HIV infection. The kinetics of association and dissociation of these peptides, and derivatives of these peptides, will be investigated in order to provide information to assist the development of drugs to treat AIDS. The KRIEGER laboratory will study cell surface scavenger receptors which bind chemically modified lipoproteins and have been implicated in atherogenesis, in the recognition and clearance of apoptotic cells and toxic and pathogenic substances, and in HDL metabolism. BIACORE instrument will be used to explore the complex binding properties of these diverse, multiligand receptors. The SAUER laboratory is engaged in studies of (i) repressor- DNA interactions, (ii) how intracellular proteases recognize specific sequences in proteins targeted for destruction and (iii) factors that influence homo versus heterodimerization of the Arc repressor and its variants.

五名 P.I. 的申请提出使用表面等离子共振对各种生物系统中特定蛋白质-蛋白质和蛋白质-核酸相互作用进行动力学和热力学分析。 RAJBHANDARY 实验室将开展真细菌和真核生物中蛋白质合成起始的研究。将使用野生型和突变型起始转移RNA来研究大肠杆菌和人类起始转移RNA以及相应的蛋白质合成起始因子IF2和eIF2之间特定相互作用的分子机制。 KHORANA 实验室将研究脊椎动物光感受器视紫红质光激活后视觉过程（敏化和脱敏）中蛋白质-蛋白质相互作用的结合和动力学常数。最初的重点将一方面是激活的受体与 G 蛋白、转导蛋白之间的相互作用，另一方面是受体与视紫红质激酶之间的相互作用。 KIM 实验室将研究来自 HIV gp41 核心的肽，这些肽被认为是 HIV 感染的抑制剂。将研究这些肽以及这些肽的衍生物的缔合和解离动力学，以便为协助开发治疗艾滋病的药物提供信息。 KRIEGER 实验室将研究与化学修饰脂蛋白结合的细胞表面清道夫受体，这些受体与动脉粥样硬化形成、凋亡细胞和有毒和致病物质的识别和清除以及高密度脂蛋白代谢有关。 BIACORE 仪器将用于探索这些不同的多配体受体的复杂结合特性。 SAUER 实验室致力于以下方面的研究：(i) 阻遏蛋白与 DNA 相互作用，(ii) 细胞内蛋白酶如何识别目标蛋白中的特定序列以进行破坏，以及 (iii) 影响 Arc 阻遏蛋白及其变体同源二聚化与异源二聚化的因素。