CHARACTERIZATION OF POLYCOMB GROUP COMPLEXES

Polycomb 基团复合物的表征

• 批准号: 6095930
• 负责人: ROBERT KINGSTON
• 金额: $ 20.62万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-01 至 2004-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6095930
• 关键词: DNA binding protein; Drosophilidae; Xenopus oocyte; chromatin; developmental genetics; gene induction /repression; homeobox genes; microarray technology; nucleosomes; protein sequence; suppressor mutations; transcription factor

DESCRIPTION (adapted from applicant's abstract): The polycomb-group (Pc-G) genes of Drosophila are required to maintain appropriate repression of genes that regulate developmental processes. Pc-G genes are conserved in mammals, and mutation of the mammalian homologs leads to severe developmental defects. It is believed that Pc-G proteins function, at least in part, by forming complexes that establish a repressive chromatin structure that maintains the 'off' state of homeotic genes. In order to investigate the mechanism of repression, it is necessary to purify Pc-G complexes and to establish assays that display the function of these complexes. This proposal describes an analysis of a Pc-G complex called PRC1 that has been isolated from Drosophila and that contains several Pc-G gene products. This analysis includes the following aims: 1) Identification of the proteins that make up PRC1. There are several unknown proteins in PRC1, and these proteins will be sequenced, their cDNAs will be isolated, and the encoded proteins will be characterized using genetic and biochemical approaches. 2) A detailed functional analysis of PRC1 will be performed. 3) The human homolog of PRC1 will be isolated and characterized. These experiments should increase the knowledge of how genes are maintained in a repressed state during development.

描述（改编自申请人的摘要）：多梳群（Pc-G） 果蝇的基因需要维持适当的基因抑制 调节发育过程。 Pc-G 基因在哺乳动物中是保守的，并且 哺乳动物同源物的突变导致严重的发育缺陷。 它 据信，Pc-G 蛋白至少部分通过形成复合物发挥作用 建立维持“关闭”状态的抑制性染色质结构 同源异型基因。 为了探究抑制机制， 纯化 Pc-G 复合物和建立显示 这些复合物的功能。 该提案描述了对 Pc-G 的分析 称为 PRC1 的复合体已从果蝇中分离出来，其中包含 几种Pc-G基因产物。 该分析包括以下目标：1) 鉴定构成 PRC1 的蛋白质。 还有几个不为人知的 PRC1中的蛋白质，这些蛋白质将被测序，它们的cDNA将被 分离，编码的蛋白质将使用遗传和 生化方法。 2) PRC1的详细功能分析将是 执行。 3) PRC1的人类同源物将被分离和表征。 这些实验应该增加人们对基因如何维持的了解。 发展过程中的压抑状态。