CORE--PROTEIN CHEMISTRY

核心--蛋白质化学

• 批准号: 6299970
• 负责人: RYUJI KOBAYASHI
• 金额: $ 37.41万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-01-01 至 2000-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6299970
• 关键词: SDS polyacrylamide gel electrophoresis; biomedical facility; high performance liquid chromatography; mass spectrometry; method development; peptide chemical synthesis; posttranslational modifications; protein purification; protein sequence; protein structure function; synthetic peptide; virus protein

The Protein Chemistry Core Component is dedicated to collaborative projects with members of this Program Project involving the structural analysis of proteins. The projects are derived from all of the research components in the Program and address problems in regulation of DNA synthesis, of programmed cell death or apoptosis, of mRNA processing, and of transcription as they relate to the oncognic transformation of cells. These projects fall into four categories: (I) proteins involved in DNA synthesis and chromatin assembly, (ii) proteins involved in apoptosis, (iii) proteins involved in mRNA splicing, and (iv) proteins involved in transcriptional control of viral and cellular genes. The methodologies involved in these investigations involve three areas: protein sequencing, analysis of protein modification, and peptide synthesis. The Core Component serves four specific function: 1. Determination of peptide sequences of purified proteins for isolation and identification of specific cDNA and genes. 2. Analysis of post-translational modifications. 3. Synthesis of peptides for functional studies and for immunizations. 4. Optimization of sensitive methods in protein chemistry.

蛋白质化学核心成分致力于协作 与该计划项目成员的项目涉及 蛋白质的结构分析。这些项目源自所有 该计划中的研究组件并解决了 调节DNA合成，程序性细胞死亡或凋亡的调节， 与mRNA处理和与转录有关的 细胞的认知转化。 这些项目分为四个 类别：（i）与DNA合成和染色质有关的蛋白质 组装，（ii）涉及凋亡的蛋白，（iii）蛋白 在mRNA剪接中，（iv）蛋白参与转录 控制病毒和细胞基因。 涉及的方法 这些研究涉及三个领域：蛋白质测序，分析 蛋白质修饰和肽合成。核心组件 提供四个特定功能：1。测定肽序列 纯化的蛋白质用于分离和鉴定特定cDNA 和基因。 2。翻译后修饰的分析。 3。 用于功能研究和免疫接种的肽的合成。 4。优化蛋白质化学敏感方法。