Core--Lipoprotein

核心--脂蛋白

• 批准号: 6925497
• 负责人: SISSEL LUND-KATZ
• 金额: $ 11.58万
• 依托单位: CHILDREN'S HOSP OF PHILADELPHIA
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6925497
• 关键词: SDS polyacrylamide gel electrophoresis; animal tissue; apolipoproteins; biomedical facility; clinical research; electron microscopy; gel electrophoresis; high performance liquid chromatography; human tissue; protein purification; ultracentrifugation; western blottings

It is the objective of this laboratory to provide plasma lipoproteins and purified apolipoproteins isolated according to carefully standardized procedures for use by the individual projects within the program. This core facility is central to the Program Project and every member utilizes it. This core has three main purposes which are: I. Lipoprotein Production a). Large-scale isolation and purification of lipoproteins and related products from normolipidemic human serum by ultracentrifugation b). Analytical preparation oflipoproteins from experimental samples (including mouse sera, dyslipidemic human sera, and tissue culture media) by FPLC II. Apolipoprotein Production Isolation of human HDL apolipoproteins apo A-I and A-II III. Characterization of Lipoprotein and Apolipoproteins Using the Following Techniques: a). Agarose gel electrophoresis of lipoproteins b). SDS-PAGE ofapolipoproteins c). Immunoblotting of apolipoproteins d). Electron microscopy of native and reconstituted lipid-protein complexes The benefits arising from the centralized service provided by the Core Laboratory include: 1) uniformity of materials and techniques; 2) optimal use of space, supplies and equipment, especially centrifuges and FPLC.

该实验室的目的是根据精心标准化的程序提供血浆脂蛋白和纯化的载脂蛋白，以供该计划中的各个项目使用。此核心设施是程序项目的核心，每个成员都利用它。该核心具有三个主要目的： I.脂蛋白产生 一个）。脂蛋白及相关产品的大规模隔离和纯化。 超离心分离的人血清 b）。来自实验样品的分析制备脂蛋白（包括小鼠血清， FPLC的血脂血清血清和组织培养基） ii。载脂蛋白的产生 隔离人HDL载脂蛋白Apo A-I和A-II iii。使用以下技术对脂蛋白和载脂蛋白的表征： 一个）。脂蛋白的琼脂糖凝胶电泳 b）。 SDS-PAGE OFORIPOPOROTIS c）。载脂蛋白的免疫印迹 D）。天然和重构脂质 - 蛋白质复合物的电子显微镜 核心实验室提供的集中服务带来的好处包括：1）材料和技术的均匀性； 2）最佳使用空间，耗材和设备，尤其是离心机和FPLC。