CORE--PROTEOMICS

核心--蛋白质组学

• 批准号: 6972210
• 负责人: THOMAS C VANAMAN
• 金额: $ 13.8万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2005-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6972210
• 关键词: SDS polyacrylamide gel electrophoresis; biomedical facility; computer program /software; high performance liquid chromatography; immunocytochemistry; mass spectrometry; matrix assisted laser desorption ionization; posttranslational modifications; protein quantitation /detection; protein sequence; proteomics; robotics; two dimensional gel electrophoresis

The proteomics core is designed to provide quantitative and qualitative protein profiling and identification analysis, as well as post-translational modification and sequence analysis through a combination of 2D gel, mass spectrometry and Edman sequencing analysis services. The core is equipped with Bio-Rad IPG and SDS-PAGE apparatus for 2D gel analysis, a Typhoon phosphorimager/scanner system for detection and quantitation of fluorescent protein dyes (Cy-dyes) and stains (SYPRO-Ruby, Pro-Q Diamond) used to analyze 1D and 2D gels, a Ciphergen SELDI-TOF protein profiling system, an ABI QSTAR II Q-TOF Mass Spectrometer with both MALDI and LC/ESI source capabilities, an ABI Procise 4 column Edman pulsed liquid phase Sequencer, Waters HPLC systems and multiple PCs for data acquisition and analysis including a site license for PDQuest software and a 5 station site license for Ciphergen software. The fully trained technical staff identified below will assist investigators with the full range of proteomics analytical services. The staff will instruct investigators and their laboratory staff (students, post-doctoral fellows and technicians) in sample preparation, 2D gel analysis and quantitation by fluorescent staining methods. Protein fragmentation, mass peptide mapping and protein sequencing by both MS/MS mass spectrometry and chemical sequencing will be performed by Dr. Beach on samples provided by individual investigators. Preparation and analysis of samples with the Ciphergen system will be facilitated by automated robotic work stations operated under the supervision of Dr. Beach. Data analysis will be performed by investigators and their colleagues through multiple distant work stations connected to the Ciphergen system via the web.

蛋白质组学核心旨在通过2D凝胶，质谱和Edman测序分析服务的组合提供定量和定性的蛋白质分析和鉴定分析以及翻译后修饰和序列分析。 The core is equipped with Bio-Rad IPG and SDS-PAGE apparatus for 2D gel analysis, a Typhoon phosphorimager/scanner system for detection and quantitation of fluorescent protein dyes (Cy-dyes) and stains (SYPRO-Ruby, Pro-Q Diamond) used to analyze 1D and 2D gels, a Ciphergen SELDI-TOF protein profiling system, an ABI QSTAR II Q-TOF具有MALDI和LC/ESI源功能的质谱仪，ABI Procise 4列Edman脉冲液相序列，Waters HPLC系统和多个用于数据获取和分析的PC PDQUEST软件的站点许可证和5个站点的CIPHERGEN软件许可证。以下确定的全面培训的技术人员将帮助调查人员提供各种蛋白质组学分析服务。工作人员将指导调查人员及其实验室工作人员（学生，博士后研究员和技术人员）进行样品制备，2D凝胶分析和荧光染色方法的定量。 Beach博士将对单个研究人员提供的样品进行蛋白质碎片化，质量肽图和蛋白质测序。自动机器人工作将促进使用Ciphergen系统的样品的准备和分析 电台在海滩博士的监督下运营。数据分析将由研究人员及其同事通过通过网络连接到Ciphergen系统的多个遥远的工作站进行。