CORE-- BIOCHEMICAL MANAGEMENT

核心——生化管理

• 批准号: 6234415
• 负责人: THOMAS C VANAMAN
• 金额: $ 1.14万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-01 至 1998-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6234415
• 关键词: adenosinetriphosphatase; biomedical facility; calmodulin; enzymes; histopathology; immunologic assay /test; laboratory rabbit; protein kinase

The Core Biochemistry Laboratory will be a major resource for the integration of the individual studies proposed in this Program Project. It will provide a uniform set of enzyme and immunoassays for samples submitted by each of the subcomponents. The Core Laboratory will also be a source of purified proteins and enzymes including calmodulin, calmodulin regulated enzymes, protein kinases and phosphatases, as well as immunoreagents for a variety of studies of individual investigations. The set of enzymes and proteins to be analyzed in all samples submitted to the Core was selected to test the hypothesis that alterations in the calcium signalling machinery are essential components in the neurodegeneration of Alzheimer's disease. Correlation of data obtained from uniform assays performed on normal and AD human tissues will test this hypothesis directly. Comparison of these results with analyses of samples from experimental animals and cultured cells treated in various ways, may permit the identification of possible factors (i.e., ischemic events, beta- amyloid production, glucocorticoid overproduction) which may trigger the biochemical alterations in Ca2+ regulation leading to neuronal cell death.

核心生物化学实验室将成为 该计划项目中提出的个别研究的整合。 它 将为提交的样品提供一组均匀的酶和免疫测定 由每个子组件。 核心实验室也将是 纯化的蛋白质和酶，包括钙调蛋白，调节钙调蛋白 酶，蛋白激酶和磷酸酶，以及用于A的免疫反应 各种研究的研究。 在提交给的所有样品中要分析的酶和蛋白质集 选择了核心来检验以下假设 钙信号机械是重要组成部分 阿尔茨海默氏病的神经变性。 获得的数据的相关性 在正常和AD人体组织上进行的均匀测定法会测试 直接假设。 这些结果与样品的分析进行比较 从以各种方式处理的实验动物和培养的细胞，可能 允许识别可能的因素（即缺血性事件，β- 淀粉样蛋白产生，糖皮质激素过量生产），这可能触发 Ca2+调节的生化改变导致神经元细胞死亡。