CORE--PROTEIN CHEMISTRY

核心--蛋白质化学

• 批准号: 6575612
• 负责人: RYUJI KOBAYASHI
• 金额: $ 22.84万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-21 至 2002-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6575612
• 关键词: DNA binding protein; DNA replication; SDS polyacrylamide gel electrophoresis; biomedical facility; cellular oncology; chromatin; complementary DNA; high performance liquid chromatography; mass spectrometry; method development; neoplastic process; peptide chemical synthesis; posttranslational modifications; protein purification; protein sequence; protein structure function; synthetic peptide; virus protein

DESCRIPTION (provided by applicant): The Protein Chemistry Core is dedicated to collaborative projects with members of this Program Project involving the structural analysis of proteins. This core will perform primary structural analysis of cellular and viral proteins involved in basic cellular processes and tumor progression. The biological problems approached by this effort include chromatin inheritance, DNA binding and replication studies in Papillomavirus, transcriptional regulation, regulation of Pre-mRNA splicing, proteolytic control of oncoprotein function, human cell transformation, oncogene-dependent apoptosis and tumor suppression. Specifically, this core component provides the following services: (1) Determination of partial peptide sequences of purified proteins for isolation and identification of specific cDNAs and genes. (2) High throughput identification of proteins by tandem mass spectrometry. (3) Analysis of post-translational protein modifications. (4) Synthetic peptides for functional analyses and immunizations.

描述（由申请人提供）：蛋白质化学核心是专用的 与该计划项目的成员合作项目，涉及 蛋白质的结构分析。该核心将执行主要结构 分析参与基本细胞过程的细胞和病毒蛋白 和肿瘤进展。这项工作解决的生物学问题 包括染色质遗传，DNA结合和复制研究 乳头瘤病毒，转录调节，调节前mRNA剪接， 癌蛋白功能的蛋白水解控制，人类细胞转化， 癌基因依赖性凋亡和肿瘤抑制。具体来说，这个核心 组件提供以下服务： （1）确定纯化蛋白的部分肽序列 特定cDNA和基因的分离和鉴定。 （2）高吞吐量 通过串联质谱法鉴定蛋白质。 （3）分析 翻译后蛋白质修饰。 （4）合成肽 功能分析和免疫。