DEVELOPMENT OF BIOPHYSICAL METHODS FOR STUDYING BIOCHEMICAL REACTIONS

研究生化反应的生物物理方法的发展

• 批准号: 3858012
• 负责人: R L BERGER
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3858012
• 关键词: Raman spectrometry; adenosine diphosphate; adenosine triphosphate; adenosinetriphosphatase; antiseptic sterilization; bioengineering /biomedical engineering; biomedical equipment development; blood /plasma substitute; calorimetry; carbon dioxide; carbon monoxide; chemical binding; chemical reaction; chlorine; hemoglobin; human tissue; infrared spectrometry; macromolecule; nucleotides; phosphates; respiratory oxygen; spectrometry; temperature

Work on the development of instrumentation for the evaluation of various hemoglobin derivatives proposed as a blood substitute has proceeded at an accelerated rate with the coming on line of the pilot plant at LAIR which can furnish large amounts of the protein. The oxygen equilibrium curve apparatus is now being tested on human hemoglobin samples. Calorimetric measurements of the binding of carbon monoxide, carbon dioxide, & chloride to HbAo and the DBBF-Hb product are underway. Progress on the use of near-infra red spectroscopy for studying ATP,ADP, & phosphate in their reactions with ATPase has proceeded to the point that a separate progress report is being written for this work. Initial Raman spectra have been taken which show differences in the phosphate moiety of the different nucleotides. A separate report on this work is also being submitted.

致力于开发各种评估仪器 提议作为血液替代品的血红蛋白衍生物已在 随着 LAIR 试点工厂的上线，速度加快了 可以提供大量的蛋白质。 氧平衡曲线 目前正在对人体血红蛋白样本进行测试。 量热法 一氧化碳、二氧化碳和的结合的测量 将氯化物转化为 HbAo 和 DBBF-Hb 产品正在进行中。 进展情况 使用近红外光谱研究 ATP、ADP 和磷酸盐 他们与 ATPase 的反应已经进行到了一个单独的 正在为这项工作编写进度报告。 初始拉曼光谱 已采取，显示出磷酸部分的差异 不同的核苷酸。 关于这项工作的单独报告也正在编制中 已提交。