DEVELOPMENT OF NIR FOR MONITORING ENZYME-SUBSTRATE REACTIONS

开发用于监测酶-底物反应的近红外光谱

• 批准号: 3858005
• 负责人: R L BERGER
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3858005
• 关键词: adenosine diphosphate; adenosine triphosphate; bioengineering /biomedical engineering; chemical reaction; enzyme mechanism; enzyme substrate complex; infrared spectrometry; phosphates

We have employed near infrared spectroscopy to quantify each of the compounds ATP,ADP, and PO 4. We have successfully made calibration curves for each in the 1 to 25 micromolar regime. Recently, mixtures of the three phosphate compounds were also quantified in the same concentration region. Hence, the distinction of ATP, ADP, and P(i) can be achieved with near infrared. Because there are significant variations in the near infrared spectra of ATP and ADP and the majority of change between ATP and ADP lies within the phosphate moiety, it follows that the differences being followed in the near infrared spectra of ADP and ATP lie within this environment.

我们采用近红外光谱来量化每一个 化合物ATP、ADP和PO 4。我们已成功制作校准曲线 每个在 1 至 25 微摩尔范围内。 最近，这三者的混合物 磷酸盐化合物也在同一浓度区域进行了定量。 因此，ATP、ADP 和 P(i) 的区分可以通过近 红外线的。 因为近红外光有很大的变化 ATP 和 ADP 的光谱以及 ATP 和 ADP 之间的大部分变化在于 在磷酸基团内，差异如下 ADP 和 ATP 的近红外光谱就位于这个环境中。