MONITOR UPTAKE, RETENTION, SUBCELL LOCALIZATION & LIFETIME OF FLUORESCENT DRUGS

监测摄取、保留、亚细胞定位

• 批准号: 6119976
• 负责人: ZBIGNIEW DARZYNKIEWICZ
• 金额: $ 0.77万
• 依托单位: UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-01 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6119976
• 关键词: biomedical resource; human tissue; nucleic acids; proteins

The uptake and binding of ellipticine was determined in viable human promyelocytic (HL-60) cells co-stained with Hoechst 33342 using a two-laser excitation flow cytometry system. Phase-sensitive FCM methods were used to measure the fluorescence lifetime of ellipticine bound within viable HL-60 cells. Ellipticine fluorescence lifetime was altered in both a time and dose dependent manner, demonstrating the disproportionate binding of ellipticine to DNA, to other cellular constituents. This study showed the utility of measuring the uptake and binding of chemotherapeutic agents in viable cell populations, and demonstrates the usefulness of fluorescence lifetime measurements. Fluorescence lifetime studies in progress are designed to compare drug uptake and binding in drug-resistant HL-60 cells and the wild-type cells.

椭圆氨酸的摄取和结合在可行 使用HOECHST 33342共同染色的人类临时细胞（HL-60）细胞使用 两个激励流式细胞仪系统。 相位敏感的FCM 使用方法来测量椭圆氨酸的荧光寿命 结合在生存的HL-60细胞内。 椭圆氨酸荧光寿命 在时间和剂量依赖性的方式上都发生了变化，证明 椭圆氨酸与DNA与其他细胞的结合不成比例 成分。 这项研究表明了测量摄取的实用性 并结合可行细胞群体中的化学治疗剂，以及 证明荧光寿命测量的有用性。 荧光寿命研究旨在比较药物 耐药的HL-60细胞和野生型的摄取和结合 细胞。