EXPRESSION OF CELL CYCLE DEPENDENT CYCLINS MEASURED BY FLOW CYTOMETRY

通过流式细胞术测量细胞周期依赖性细胞周期蛋白的表达

• 批准号: 6470667
• 负责人: ZBIGNIEW DARZYNKIEWICZ
• 金额: $ 12.12万
• 依托单位: UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6470667
• 关键词: animal tissue; biomedical resource; biotechnology; liver

The cyclins are a family of cell cycle-related proteins that are expressed at different times and are involved in regulation of cells through the various phases of the cell cycle. In general, cyclins D and E appear primarily during G1 phase, cyclin A during S and cyclin B in G2 phase. The cyclins complex with cyclin-dependent kinases and these complexes phosphorylate proteins that are important for traverse of cells through the cell cycle. Antibodies are now available to the major cyclins and utilization of these probes for analysis of cyclin expression provides an important tool for measuring the position and frequency of cells in the various phases of the cell cycle. Darzynkiewicz and co-workers have developed and utilized cell-labeling and FCM techniques to demonstrate the correlation of cyclin D and E expression and staurosporine-induced G1 arrest of human lymphocytes and the effects of G2 arrest and polyploidization of transformed cells and cyclin B. U tilizing similar method we are investigating the expression of cyclins on human diploid fibroblasts following exposure to alpha and gamma irradiation.

细胞周期蛋白是一个与细胞周期相关的蛋白质家族 在不同的时间表达，并参与细胞的调节 通过细胞周期的各个阶段。 通常，细胞周期蛋白D E主要出现在G1阶段，在S期间Cyclin A和Cyclin B 在G2阶段。 与细胞周期蛋白依赖性激酶和 这些复合物磷酸化蛋白对于遍历很重要 整个细胞周期的细胞。 抗体现在可用于 主要的细胞周期蛋白和这些探针的利用来分析细胞周期蛋白 表达提供了测量位置和的重要工具 细胞在细胞周期的各个阶段的频率。 Darzynkiewicz和同事已经开发和使用了细胞标记 和FCM技术以证明细胞周期蛋白D和E的相关性 表达和星形孢菌素诱导的G1人类淋巴细胞停滞 以及转化细胞的G2停滞和多倍化的影响 和Cyclin B. u化类似方法，我们正在研究 暴露后，细胞周期蛋白在人二倍体成纤维细胞上的表达 到Alpha和Gamma辐照。