BIACORE 2000

比亚科2000

• 批准号: 2472760
• 负责人: LAURENCE H KEDES
• 金额: $ 23.5万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-03-01 至 1999-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2472760
• 关键词: biomedical equipment; biomedical equipment purchase; biosensor device; intermolecular interaction; surface plasmon resonance

The Biosensor BIAcore instrument will enable us to directly observe and measure the binding affinities and association/dissociation kinetics of molecular interactions in real time, thus greatly accelerating the process of identifying and characterizing both protein-protein and protein-DNA interactions. The BIAcore instrument contains an immobilized ligand on a sensor chip, and can monitor the adsorption of unlabelled proteins in free solution to the chip by means of surface plasmon resonance detection, that is, changes in the refractive index in the vicinity of the surface of the chip. These changes are directly proportional to the change in adsorbed mass, enabling characterization of biomolecular interactions. The bound material can also be eluted and recovered from the sensor chip, thus allowing identification of previously unknown molecular interactions. This instrument can in fact be used to screen phage display libraries which express scFv sequences, as well as characterize known protein-protein or proteinDNA interactions. The major users of this instrument are all NIH grant recipients and will constitute 80% of the estimated usage. Their projects represent a diverse array of interests that reflect the flexible and highly useful nature of the instrument, and include examination of protein:DNA and protei-protein interaction that regulate transcriptional activation in hypertension, stress, cell cycle, and hormonal signal transduction, as well as mechanisms of vrial envelope binding to cellular surface receptors. The minor users of this instrument represent 20% of the estimated usage, and consist of junior faculty who have not yet received NIH funding; their projects also illustrate the diverse applications to which this instrument can be adapted, including characterization of RNA binding protein interactions with oncogene transcripts, and screening of phage display libraries to obtain cell surface binding peptides and single chain antibodies with unique specificities.

生物传感器biacore仪器将使我们能够直接观察和 测量结合亲和力和关联/解离动力学 实时分子相互作用，因此大大加速了过程 鉴定和表征蛋白质 - 蛋白质和蛋白-DNA 互动。 Biacore仪器在A上含有固定的配体 传感器芯片，可以监测自由中未标记蛋白的吸附 通过表面等离子体共振检测来解决芯片， 是，在表面附近折射率的变化 芯片。这些变化与吸附的变化成正比 质量，可以表征生物分子相互作用。绑定 材料也可以从传感器芯片中洗脱并回收，因此 允许鉴定以前未知的分子相互作用。这 实际上，可以使用仪器筛选噬菌体显示库 表达SCFV序列，并表征已知的蛋白质蛋白质或 Proteindna相互作用。 该工具的主要用户都是NIH赠款收件人，将 构成估计用法的80％。他们的项目代表了多元化 一系列兴趣，反映了灵活和非常有用的性质 仪器，包括蛋白质的检查：DNA和蛋白质蛋白 调节高血压转录激活的相互作用， 应力，细胞周期和激素信号转导，以及 Vrial包膜与细胞表面受体结合的机理。这 该工具的次要用户占估计用法的20％，并且 由尚未获得NIH资金的初级教师组成；他们的 项目还说明了该工具的各种应用 可以改编，包括RNA结合蛋白的表征 与癌基因转录本的相互作用，并筛选噬菌体显示 文库获得细胞表面结合肽和单链 具有独特特异性的抗体。