NCRR Shared Instrumentation Grant (SIG) Program

NCRR 共享仪器资助 (SIG) 计划

• 批准号: 6879448
• 负责人: MICHAEL B YAFFE
• 金额: $ 32.84万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-01-01 至 2005-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6879448
• 关键词: biomedical equipment purchase; biosensor device; cryopreservation; imaging /visualization /scanning; optics

DESCRIPTION (provided by applicant): In this proposal, four NIH-funded faculty members in the Department of Biology of the Massachusetts Institute of Technology request funding to replace much of the aging equipment of the department's Structural Biology Core Facility. The requested funding will be used to upgrade this dilapidated facility into a state-of-the-art, multi-user core facility capable of serving the needs of the principal investigators and other members of the MIT structural biology community for many years. The proposed upgrade would replace the old generator, optics and cryo-system with modern instruments. The R-AXIS IV detector currently in use would be maintained and used with the new, equipment. The specific instruments requested in this proposal are a Rigaku MicroMax 007 generator, an Oxford Cryosystems CSCryostream700EX sample cooler, and Rigaku/Osmic VariMax Cu optics. The old generator needs replacing because it rapidly approaching the end of its useful lifetime. More importantly, the new generator can provide about twice the useable flux for the typical frozen crystal. Combined with the new brighter source, the new, vastly superior, optics will dramatically improve the facility's capability to collect high quality data from small or weakly diffracting crystals and significantly increase the overall capacity of the system to generate crystallographic data. The new cryo-system is desperately needed because the performance of old system has become, at best, marginal. The new equipment will replace existing instrumentation in the Facility on the fifth floor of the Koch Biology Building, where it is readily accessible to all of the primary users and the MIT biology community as a whole. The new instrumentation will dramatically improve the productivity of principal investigators' structural biology research, provide capability not currently available anywhere at on campus, and contribute to providing the best possible training of the next generation of structural biologist from MIT.

描述（由申请人提供）：在此提案中，马萨诸塞州理工学院生物学系的四名NIH资助的教职员工要求提供资金，以取代该部门结构生物学核心设施的大部分老化设备。要求的资金将用于将这项破旧的设施升级为最先进的多用户核心设施，该设施能够满足多年来主要研究人员和MIT结构生物学社区的其他成员的需求。拟议的升级将用现代仪器代替旧的发电机，光学和冷冻系统。当前正在使用的R轴IV检测器将与新设备一起维护和使用。本提案中要求的特定仪器是Rigaku Micromax 007发电机，牛津冷冻系统CSCRyoStream700EX样品冷却器和Rigaku/Osmic varimax Cu光学器件。旧发电机需要更换，因为它迅速接近了其有用的寿命的尽头。更重要的是，新发电机可以为典型的冷冻晶体提供约两倍的可用通量。结合新的更明亮的来源，新的，非常出色的光学器件将大大提高该设施从小型或弱衍射的晶体收集高质量数据的能力，并显着增加系统的总体容量来生成晶体学数据。迫切需要新的冷冻系统，因为旧系统的性能充其量已经变得很边缘。新设备将取代科赫生物学大楼五楼的设施中的现有仪器，所有主要用户和整个MIT生物学社区都可以访问它。新仪器将大大提高主要研究人员结构生物学研究的生产率，提供当前在校园任何地方都无法获得的能力，并为MIT提供了下一代结构生物学家的最佳培训。