ACQUISITION OF A 400 MHZ NMR SPECTROMETER FOR RESEARCH

购买 400 MHZ 核磁共振波谱仪用于研究

基本信息

批准号：
2503787
负责人：
BRUCE GANEM
金额：
$ 33万
依托单位：
CORNELL UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1998
资助国家：
美国
起止时间：
1998-05-20 至 1999-05-19
项目状态：
已结题

项目摘要

This proposal is a request for the funds required to purchase a 400 MHz NMR spectrometer to expand and update current capabilities in NMR instrumentation at Cornell University. The requested spectrometer will be devoted to structural studies of small molecules in liquids and will provide modern techniques to a core group of NIH-supported users. The spectrometer will be housed in the Chemistry Department NMR Facility, which provides instrumentation and training to almost 200 students and researchers from the university community and local industry. Five NIH- funded Cornell faculty members require access to this spectrometer to carry out a variety of health-related projects. Professor B. Ganem (Principal Investigator) will study (1) potential inhibitors of chorismate mutase/prephenate dehydratase involved in the shikimic acid pathway, (2) the synthesis of glycosidase inhibitors, (3) the synthesis of purine nucleoside phosphorylase inhibitors important for the treatment of AIDS, and (4) inhibitors of glutathione and trypanothione reductases. Professor T. Begley will investigate enzyme function and mechanism by using NMR spectroscopy to elucidate the structure of products of enzyme-catalyzed reactions. These reactions include the (6) thiamin biosythetic pathway, (7) enzymatic repair of UV-irradiated DNA in bacterial spores, (8) the mechanism and inhibition of prolyl-4- hydroxylase, and (9) the biosynthesis of taxol. Professor J. Clardy will investigate natural products from novel sources including (10) anticancer compounds isolated from endophytic fungi and (11) compounds displaying antibiotic properties toward the plant pathogens responsible for fire blight. Professor D. B. Collum will investigate (5) organolithium solvation and aggregation using 6 Li-detected triple- resonance spectroscopy. Professor J. Meinwald will continue to pioneer the field of chemical ecology by exploring (12) biologically active compounds isolated from insect sources.

该提案是请求购买 400 MHz 所需的资金 NMR 波谱仪可扩展和更新 NMR 的当前功能康奈尔大学仪器仪表。所要求的光谱仪将致力于液体中小分子的结构研究，并将为 NIH 支持的核心用户群体提供现代技术。这光谱仪将安装在化学系核磁共振设施中，为近 200 名学生提供仪器和培训来自大学社区和当地工业界的研究人员。五个 NIH- 受资助的康奈尔大学教职员工需要使用该光谱仪开展各类健康相关项目。 B.加内姆教授（首席研究员）将研究 (1) 的潜在抑制剂参与莽草酸的分支酸变位酶/预苯酸脱水酶途径，（2）糖苷酶抑制剂的合成，（3）合成嘌呤核苷磷酸化酶抑制剂的重要作用治疗艾滋病，以及（4）谷胱甘肽和锥硫酮抑制剂还原酶。 T. Begley 教授将研究酶的功能并通过使用核磁共振波谱来阐明其结构的机制酶催化反应的产物。这些反应包括 (6) 硫胺素生物合成途径，(7) 紫外线照射 DNA 的酶促修复在细菌芽孢中，（8）prolyl-4-的作用机制及抑制作用羟化酶，(9)紫杉醇的生物合成。 J·克拉迪教授将研究新来源的天然产物，包括 (10) 从内生真菌中分离出的抗癌化合物和（11）化合物对负责的植物病原体表现出抗生素特性用于火疫病。 D. B. Collum 教授将进行调查 (5) 使用 6 Li 检测的三重有机锂溶剂化和聚集共振光谱。 J. Meinwald 教授将继续开拓化学生态学领域通过探索（12）生物活性从昆虫来源中分离出的化合物。