600 MHz Nuclear Magnetic Resonance Spectrometer

600 MHz 核磁共振波谱仪

基本信息

批准号：
7047279
负责人：
DANIEL S SEM
金额：
$ 50万
依托单位：
MARQUETTE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-09-30 至 2006-09-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7047279
关键词：
NAD(H) phosphate biomedical equipment purchase nuclear magnetic resonance spectroscopy proteomics

项目摘要

DESCRIPTION (provided by applicant): A high field multinuclear NMR spectrometer is needed to support new and ongoing projects at Marquette University and the greater Milwaukee area. The proposed instrument will support and extend existing NIH and other funded projects for 6 major users and 4 minor users from 3 institutions. The major user group is funded by 8 NIH grants, and minor users are funded from various sources. The major users are involved in projects directed to problems in biochemistry that make central use of NMR, and the minor users have projects that rely heavily on NMR in a supporting role. A key feature required of the spectrometer will be the high sensitivity and resolution available at 600 MHz, in order to study protein-ligand interactions. The NMR facility will offer a wide variety of probe choices, including MicroFlow and BB, thereby satisfying a regional need unmet by other facilities. Projects include: (1) Probing protein-ligand interactions in chemical proteomics: NMR methods development and application (Sem), (2) Complementary use of NMR and Raman studies to probe ligand binding to cytochrome P450cam (Kincaid), (3) Role of Su e information of the Fi-Fo ATP synthase dimeric complex: structural and binding studies (Stuart), (4) Mechanism of flagellar radial spoke: ligand binding and structural studies of radial spoke protein 2 (RSP2) (Yang), (5) Structural characterization of molecular motions in NADPH-dependant cytochrome P450 oxidoreductase (Kim) and (6) Substrate binding geometries in Fell dependent non-heme oxygenases from paramagnetic relaxation and heteronudear correlation (Moran). The spectrometer is the center-piece of the "Chemical Proteomics Facility at Marquette", and accordingly has the strong support of Marquette University which has committed the additional $368,945 needed beyond the $500,000 being requested, as well as needed space, facilities, staff, cryogens, warranties and other support. Although neighboring NMR facilities are currently being used, they cannot meet existing demand due largely to the need for travel of > 100 miles and advance scheduling for routine high-field experiments. Such an instrument would therefore accelerate already funded (predominantly NIH) projects across departments and institutions, as well as extend their scope. It will also better leverage the use of regional facilities for follow-up refinement studies, rather than routine early-stage experiments.

描述（由申请人提供）：需要高场多核NMR光谱仪来支持马奎特大学和大密尔沃基地区的新项目。拟议的工具将为来自3个机构的6个主要用户和4位次要用户提供支持并扩展现有的NIH和其他资助的项目。主要的用户组由8个NIH赠款资助，未成年人从各种来源资助。主要用户参与了针对NMR中心使用生物化学问题的项目，次要用户的项目严重依赖NMR担任支持角色。光谱仪所需的关键特征将是在600 MHz下可用的高灵敏度和分辨率，以研究蛋白质 - 配体相互作用。 NMR设施将提供多种探针选择，包括微流和BB，从而满足其他设施未满足的区域需求。项目包括：（1）化学蛋白质组学中的蛋白质 - 培养基相互作用：NMR方法的开发和应用（SEM），（2）互补使用NMR和Raman研究以探测配体与细胞色素p450CAM（Kincaid）（Kincaid）（Kincaid）（KincAID）的结合，（KINCAID）（KINCAID），（3）FII-fo atp synthase和Synthase dimeric dimeric dimeric dimertrarrar的su信息的作用（结构化）：结构化合物：结构化，结构化，结构化，结构化，结构化，结构化，结构化，结构化。径向辐条：径向辐条蛋白2（rsp2）（yang）的配体结合和结构研究，（5）NADPH依赖性细胞色素p450氧化还原酶（KIM）和（6）在有依赖的非阳性氧化摩我的氧化摩我的副氧化摩我的NADPH依赖性细胞氧化还原酶（KIM）和（6）副氧结合的结构表征。光谱仪是“ Marquette的化学蛋白质组学设施”的中心件，因此，Marquette University的大力支持，该大学承担了要求额外的368,945美元，仅需要求提供500,000美元的$ 500,000，以及所需的空间，设施，工作人员，员工，冷冻机构，保修和其他支持。尽管目前正在使用邻近的NMR设施，但由于需要进行> 100英里的旅行需要并进行日程安排，因此它们无法满足现有需求。因此，这种工具将加速已经为部门和机构提供的已经资助的（主要是NIH）项目，并扩大其范围。它还将更好地利用区域设施用于后续改进研究，而不是常规的早期实验。