CORE--NEUROPATHOLOGY

核心--神经病理学

• 批准号: 5204440
• 负责人: LAWRENCE A. HANSEN
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5204440
• 关键词: Alzheimer's disease; Lewy body; biomedical facility; brain; brain disorder diagnosis; confocal scanning microscopy; cryopreservation; disease /disorder classification; histopathology; human tissue; image processing; immunocytochemistry; neuritic plaques; neurochemistry; neurofibrillary tangles; postmortem; tissue resource /registry

The neuropathology core is maintained by a professional staff of Lawrence Hansen, M.D. and Eliezer Masliah, M.D. Its functions include neuropathologic and neurochemical analyses of autopsy specimens from patients clinical evaluated at the ADRC, and maintenance of a brain tissue bank of frozen and fixed material from such clinically and neuropathologically characterized patients. On the basis of the past several years experience, we anticipate receiving 50-60 brains annually, mostly originating from the ADRC. In may cases, rapid autopsies (within 8 hours of death) allow fresh brain tissues to be distributed to ADRC affiliated research laboratories for use in RNA biochemistry, in situ hybridization/autoradiography, and immunohistochemistry. After removing the appropriate fresh tissue blocks for such studies, the right hemibrain is frozen at 70. This specimen is utilized for neurochemical measurements of choline acetyltransferase, somatostatin, and synaptophysin, as well as for western blot analyses to determine antibody sensitivities and specificities for correlations with various immunohistochemical studies. Frozen brain tissue is also available for distribution to other ADRC investigators. Neuropathologic studies are carried out on the formalin fixed left hemibrain, and involve multiple stains on multiple blocks from all parts of the brain. Diagnoses are established by the location and concentration of plaques and tangles as quantified with thioflavin and Bielschowsky preparations. We will also neuropathologically stage the extent of Alzheimer disease pathology in Alzheimer cases and in our elderly controls using the staging classification of Braak and Braak. Other diagnoses are added or ruled- out. Lewy body disease is evaluated with both H&E and anti-ubiquitin immunohistochemical preparations. Cresyl violet stained section are used for neocortical cell counting by image analysis. All data are recorded in the computerized data base of this core, as well as in the ADRC main computer.

神经病理学核心由劳伦斯的专业人员维护 医学博士Hansen和M.D. Eliezer Masliah的职能包括 来自尸检标本的神经病理和神经化学分析 患者在ADRC上评估并维持大脑 从临床和 神经病理学特征的患者。 基于过去 几年的经验，我们预计每年会收到50-60个大脑， 主要来自ADRC。 在5月的情况下，快速尸检（内部 8小时死亡）允许将新鲜的脑组织分布到ADRC 隶属的研究实验室用于RNA生物化学，原位 杂交/放射自显影和免疫组织化学。 删除后 适当的新鲜组织块用于此类研究，右半纤维 在70时冷冻。该标本用于神经化学 测量胆碱乙酰转移酶，生长抑素和 突触素以及蛋白质印迹分析以确定抗体 与各种相关性的敏感性和特异性 免疫组织化学研究。 冷冻脑组织也可用于 分配给其他ADRC调查人员。神经病理学研究是 在福尔马林固定的左半纤维上进行，涉及多个 来自大脑各个部分的多个块上的污渍。 诊断是 由斑块和缠结的位置和浓度建立为 用硫非类和Bielschowsky制剂量化。 我们也会 神经病理学上的阿尔茨海默氏病病理学的程度 阿尔茨海默氏症病例和我们的老年对照中使用该分期 Braak和Braak的分类。 添加或裁定其他诊断 - 出去。 H＆E和抗泛素均评估Lewy身体疾病 免疫组织化学制剂。 使用甲状腺紫外线染色部分 用于新皮细胞通过图像分析计数。 所有数据均记录 在该核心的计算机数据库中以及ADRC Main中 电脑。