CORE--NEUROPATHOLOGY

核心--神经病理学

• 批准号: 6395442
• 负责人: LAWRENCE A. HANSEN
• 金额: $ 0.92万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-15 至 2000-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6395442
• 关键词: Alzheimer's disease; Lewy body; alpha synuclein; biomedical facility; brain; brain disorder diagnosis; choline acetyltransferase; cryopreservation; disease /disorder classification; histopathology; human tissue; image processing; immunocytochemistry; neuropathology; postmortem; stainings; tissue /cell preparation; tissue resource /registry; ubiquitin

The neuropathology core is maintained by a professional staff of Lawrence A. Hanson, M.D. and Eliezer Masliah, M.D. The core will provide post- mortem diagnoses on brain from demented and normal aged control patients clinically evaluated by the San Diego ADRC and maintain a brain tissue bank of formalin fixed and frozen brain tissue from such clinically and neuropathologically characterized patients. Diagnoses will be assigned in accordance with the new neuropathological criteria recently developed the NIA-Regan Institute Working Group. More detailed neuropathologic profiling on each specimen for research purposes will also entail quantification of neocortical and hippocampal plaques and tangles, immunohistochemical stains for amyloid, tau, and ubiquitin, and Braak staging. On the basis of past experience, we anticipate receiving 40 brains annually. In some cases, rapid autopsies (within 8 hours of death) allow fresh brain tissue to be distributed to ADRC affiliated research laboratories for use in RNA biochemistry, in situ section in the midline with the neuropathologic evaluation of the left hemibrain and freezing of the right hemibrain for distribution of frozen brain tissue to other investigators within and outside the ADRC. The standard neuropathological protocol includes H&E, thioflavin-S, anti-amyloid, anti-tau, and anti-ubiquitin preparations of frontal, temporal and parietal lobes, anterior and posterior hippocampus, substantia innominata, mesencephalon, and pons. Lewy body disease is evaluated with both H&E and anti-ubiquitin immunohistochemical preparations. Cresyl violet stained sections are used for neocortical morphometry and image analysis. ChAT activity and synaptophysin measurements are performed to support specific projects. All data are recorded in the computerized database of this core as well as in the ADRC main computer, and can be shared with other ADDCC centers upon request.

神经病理学核心由劳伦斯·A·汉森（Lawrence A.诊断将根据最近开发的Nia-Regan Institute工作组的新神经病理标准分配。每个标本上的更详细的神经病理分析还需要定量新皮层和海马斑块和缠结，淀粉样蛋白，TAU和泛素和泛素的免疫组织化学染色，以及braak分期。根据过去的经验，我们预计每年将获得40个大脑。在某些情况下，快速尸检（死后8小时之内）允许将新鲜的脑组织分配给ADRC隶属的研究实验室，用于RNA生物化学，原位部分，与左半元素的神经病理评估，对左半元素的神经病理评估，并将右下摆的右侧冻结和右侧的脑组织内部和外部调查人员分配给其他研究员，以分配到其他调查人员中。标准的神经病理学方案包括H＆E，硫素S-S，抗淀粉样蛋白，抗TAU和额叶，颞叶和顶叶的抗泛素制剂，前和后海马，海马，黑质，Innominata，Mesencephalon和Pons。通过H＆E和抗泛素免疫组织化学制剂评估Lewy身体疾病。甲状腺紫色染色部分用于新皮质形态计量学和图像分析。进行聊天活动和突触素测量以支持特定项目。所有数据都记录在该核心的计算机数据库以及ADRC主计算机中，并且可以根据要求与其他AddCC中心共享。