MASS SPECTROMETRY OF DRUGS, NATURAL PRODUCTS, PROTEINS, AND OLIGONUCLEOTIDES

药物、天然产物、蛋白质和寡核苷酸的质谱分析

• 批准号: 5202053
• 负责人: L K PANNELL
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5202053
• 关键词: analytical chemistry; biological products; biomedical equipment resource; chemical structure; drug /agent; electrospray ionization mass spectrometry; liquid chromatography; mass spectrometry; oligonucleotides; protein structure; trace elements; zonal electrophoresis

Specialized mass spectrometry analyses are provided to the laboratory and to other collaborating units. The emphasis of this work is split between identification of trace organic compounds isolated from biological sources and the mass spectrometry of biopolymers (e.g. peptides, proteins and oligonucleotides). A very close working relationship and collaboration is maintained with the Laboratory of Biophysical Chemistry, NHLBI, especially with the research into, and use of techniques for, mass spectrometry of macromolecules. Approval has been received to upgrade the electrospray source on the JEOL SX1O2 instrument to enhance stability and sensitivity, and to facilitate interfacing to liquid chromatographic methods. This will be used as part of the Unit's effort into post-translational modifications of proteins. The capillary zone electrophoresis instrument continues to receive heavy usage from this laboratory and an investigator from BEIP. Use is made of the NHLBI laser desorption mass spectrometer for the analysis of proteins, protein digests and oligonucleotides as part of our growing work in biopolymer mass spectrometry. Using a combination of this instrument and the JEOL mass spectrometer, methods have been developed and applied for the determination and identification of disulfide bonds in proteins, and for the analysis of oligonucleotides and DNA by mass spectrometry. In the small molecule area, collaborative interest has continued in the identification of biologically active natural products, especially those of interest in AIDS research and treatment. Samples analyzed derive from many facilities and researchers and approximately 2600 samples were analyzed on the JEOL and over 2000 on the MALDI instrument.

专门的质谱分析提供给实验室和 到其他协作单位。 这项工作的重点是在 从生物来源分离的痕量有机化合物的鉴定 以及生物聚合物的质谱法（例如肽，蛋白质和 寡核苷酸）。 非常紧密的工作关系和协作是 与生物物理化学实验室保持NHLBI，特别是 随着技术的研究和使用，用于质谱的技术 大分子。 已获得批准以升级电喷雾 JEOL SX1O2仪器上的来源，以增强稳定性和灵敏度， 并促进与液相色谱方法的接口。 这 将用作单位努力的一部分进行翻译后 蛋白质的修饰。 毛细管电泳仪器 该实验室和调查员继续收到大量使用 来自肉。 使用NHLBI激光解吸质谱仪 为了分析蛋白质，蛋白质消化和寡核苷酸作为一部分 我们在生物聚合物质谱法中不断增长的工作。 使用组合 在该仪器和JEOL质谱仪中，方法已经 开发并应用于确定和识别 蛋白质中的二硫键，以及用于分析寡核苷酸和 DNA通过质谱法。 在小分子区域，合作 在鉴定生物学活性的情况下，兴趣继续 天然产品，特别是对艾滋病研究感兴趣的产品和 治疗。分析的样本来自许多设施和研究人员 在JEOL上分析了大约2600个样品，并在2000年以上。 马尔代仪器。