MASS SPECTROMETRY OF DRUGS, NATURAL PRODUCTS, PROTEINS, AND OLIGONUCLEOTIDES

药物、天然产物、蛋白质和寡核苷酸的质谱分析

• 批准号: 3776954
• 负责人: L K PANNELL
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3776954
• 关键词: biological products; drug /agent; electrospray ionization mass spectrometry; mass spectrometry; oligonucleotides; organic chemicals; proteins; trace elements; zonal electrophoresis

Specialized mass spectrometry analyses are provided to the laboratory and to other collaborating units. The emphasis of this work is split between identification of trace organic compounds isolated from biological sources and the mass spectrometry of biopolymers (e.g. peptides, proteins and oligonuceotides). A very close working relationship and collaboration is maintained with the Laboratory of Biophysical Chemistry, NHLBI, especially with the research into, and use of techniques for, mass spectrometry of macromolecules. The electrospray source on the JEOL SX102 instrument was enhanced by the addition of a camera which allows easy optimization of the spray during analyses. The capillary zone electrophoresis instrument continues to receive heavy usage from this laboratory and others in the Institute. This has also been interfaced to the electrospray source where its separation efficiency far surpasses that of traditional HPLC methods. As part of the macromolecule collaboration with LBC, NHLBI, routine use is made of a mass spectrometer especially set up for sequencing of protein digests. In addition, use is made of a Laser Desorption mass spectrometer in NCI and in LBC, NHLBI (Dr. Fales) for the analysis of proteins and protein digests as part of our expansion into biopolymer mass spectrometry. In the small molecule area, collaborative interest has continued in the identification of biologically active natural products, especially those of interest in AIDS research and treatment; several anticonvulsant plasma drug level studies have been completed in collaboration with NINCDS. Samples analyzed derive from many facilities and researchers.

专门的质谱分析提供给实验室和 到其他协作单位。 这项工作的重点是在 从生物来源分离的痕量有机化合物的鉴定 以及生物聚合物的质谱法（例如肽，蛋白质和 寡素化剂）。 非常紧密的工作关系和协作是 与生物物理化学实验室保持NHLBI，特别是 随着技术的研究和使用，用于质谱的技术 大分子。 JEOL SX102仪器上的电喷雾源是 通过添加相机来增强，该相机可以轻松优化 分析过程中的喷雾。 毛细管电泳仪器 继续从该实验室和其他人中收到大量使用 研究所。 这也已连接到电喷雾来源 它的分离效率远远超过了传统的HPLC方法。 作为与NHLBI的大分子合作的一部分，常规使用是 由质谱仪制成，特别是用于测序的蛋白质 消化。 另外，使用激光解吸质谱仪 在NCI和LBC中，NHLBI（FARES博士）用于分析蛋白质和 蛋白质消化是我们扩展到生物聚合物质谱法的一部分。 在小分子区域，合作兴趣一直在 鉴定生物活性天然产品，尤其是 对艾滋病研究和治疗的兴趣；几种抗惊厥药物药物 水平研究已与NINCDS合作完成。 样品 分析来自许多设施和研究人员。