MASS SPECTROMETRY OF DRUGS, NATURAL PRODUCTS, PROTEINS, AND OLIGONUCLEOTIDES

药物、天然产物、蛋白质和寡核苷酸的质谱分析

• 批准号: 2573684
• 负责人: L K PANNELL
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2573684
• 关键词: analytical chemistry; biological products; biomedical equipment resource; chemical structure; disulfide bond; double stranded RNA; drug /agent; electrospray ionization mass spectrometry; laser spectrometry; mass spectrometry; method development; oligonucleotides; polymers; protein structure; structural biology; trace elements

Specialized mass spectrometry analyses are provided to the laboratory and to other collaborating units. The emphasis of this work is split between identification of trace organic compounds isolated from biological sources and the mass spectrometry of biopolymers (e.g. peptides, proteins and oligonucleotide). A very close working relationship and collaboration is maintained with the Laboratory of Biophysical Chemistry, NHLBI, especially with the research into, and use of techniques for, mass spectrometry of macromolecules. While a large number of analyses are performed to assist others in their research (total analyses for current year were 3030 on the SX102 and approx. 1500 by MALDI), as much emphasis as possible is placed on mass spectrometry technique related research. Major research accomplishments have involved the development of a method to allow the first spectra of intact double-stranded and triple-stranded DNA by matrix assisted laser desorption mass spectrometry to be observed, and the identification of the disulfide bonds in three proteins. In one of the proteins, a method was developed which allowed for the identification of the cross-linking using the combined information from the many fragments formed under acid hydrolysis. Techniques for the location and identification of other post-translational modifications are under development. In collaboration with the National Institute of Standards and Technology (NIST), mass spectra of over 2400 compounds, the structures of which had been elucidated at NIH, the Walter Reed Army Institute of Research and other government agencies, have been obtained and processed for introduction to the NIST mass spectra library database. During the year, the JEOL SX102 mass spectrometer, on which most of the analyses are performed, was upgraded with a new data system and electrospray source. This has greatly enhanced the group's ability to perform biopolymer mass spectrometry analyses.

提供专门的质谱分析向实验室提供 以及其他合作单位。 这项工作的重点是分裂的 从从中分离出的痕量有机化合物的鉴定 生物来源和生物聚合物的质谱法（例如 肽，蛋白质和寡核苷酸）。 一个非常紧密的工作 关系和协作与实验室保持 生物物理化学，NHLBI，尤其是研究和 用于大分子的质谱技术的技术。 而a 进行大量分析以帮助他人 研究（当年的总分析为3030，SX102和 大约1500由Maldi），尽可能多的重点放在质量上 光谱技术相关研究。 主要研究 成就涉及开发一种方法，以允许 完整双链和三链DNA的第一光谱 矩阵辅助激光解吸质谱法可以观察到，并 三种蛋白质中二硫键的鉴定。 一个 在蛋白质中，开发了一种方法 使用组合信息识别交联 从在酸水解下形成的许多片段。 技术 其他后翻译的位置和识别 正在开发修改。与 国家标准技术学院（NIST），质谱 超过2400种化合物，其结构已在 NIH，沃尔特·里德陆军研究所和其他政府 已经获得并处理了代理机构 NIST质谱库数据库。 在这一年中，JEOL SX102质谱仪，大多数 进行分析是进行了新的数据系统升级的， 电喷雾来源。 这极大地增强了小组的能力 执行生物聚合物质谱分析。