CELL MEDIATED IMMUNITY TO HAPTEN MODIFIED SYNGENEIC LYMPHOCYTES IN MICE

小鼠中针对半抗原修饰的同源淋巴细胞的细胞介导的免疫

• 批准号: 4691755
• 负责人: G M SHEARER
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4691755
• 关键词: T lymphocyte; autologous transplantation; cell cell interaction; cell mediated cytotoxicity; cell population study; cell type; cellular immunity; clone cells; cross immunity; genetic regulation; genetic strain; haptens; helper T lymphocyte; histocompatibility gene; immune tolerance /unresponsiveness; immunogenetics; immunotoxicity; major histocompatibility complex; membrane activity; monoclonal antibody; nitrobenzene; spleen; surface antigens

Mouse spleen cells were modified with trinitrobenezene sulfonate (TNP), and the TNP-self modified cells were tested in two different ways. First, several monoclonal antibodies (mAbs) specific for class I H-2 antigens were tested for binding to TNP-modified spleen cells. Second, the modified cells were used as stimulator and target cells for in vitro tests for cytotoxic T lymphocyte (CTL) responses to TNP-self. A number of anti-H-2Kk antibodies not mAbs of other specificities exhibited enhanced binding to cells that normally express Kk. Furthermore, these same mAbs bound to H-2b cells modified with TNP. These results parallel the patterns of preferential CTL recognition in H-2k mice and of crossreactive CTL in H-2b mouse strains.

小鼠脾细胞用三硝基苯磺酸盐（TNP）修饰，并 以两种不同的方式测试了TNP自我修饰的细胞。 第一的， 对I类H-2抗原特异的几种单克隆抗体（mAb）是 测试与TNP修饰的脾细胞结合。 其次，修改后 细胞用作刺激剂和靶细胞进行体外测试 细胞毒性T淋巴细胞（CTL）对TNP自身的反应。 许多反H-2KK 抗体不是其他特异性的mab，表现出增强的结合 通常表达KK的细胞。 此外，这些相同的mAb与H-2B结合 用TNP修饰的细胞。 这些结果与 H-2K小鼠和H-2B中的交叉反应性CTL的优先CTL识别 小鼠菌株。