IMMUNE STUDIES IN HOMOSEXUAL MEN AT RISK FOR ACQUIRED IMMUNE DEFICIENCY SYNDROME

对有获得性免疫缺陷综合症风险的男同性恋者进行免疫研究

• 批准号: 3939243
• 负责人: G M SHEARER
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3939243
• 关键词: AIDS; T lymphocyte; antiAIDS agent; antiviral antibody; cellular pathology; disease /disorder proneness /risk; helper T lymphocyte; homosexuals; human immunodeficiency virus 1; immunopathology; interferons; leukocytes; male; microorganism disease chemotherapy; microorganism immunology; pyrimidine analog; serology /serodiagnosis; suppressor T lymphocyte; surface antigens; thymidine; tissue /cell culture; virus classification

Peripheral blood leukocytes (PBL) from HTLV-III antibody (+) and (-) donors were tested for proliferative (3H) and cell-mediated lympholysis (CML) to influenza virus (S+X) and to HLA allogeneic cells (ALLO). Among antibody (+) donors, approximately 50% failed to respond to S+X, whereas all responded to ALLO, except patients in the critical stages of AIDS, who responded to neither type of immunogen. Using PBL from antibody (-) donors we demonstrated by cell fractionation techniques that S+X T cell responses are obliged to use CD4+ T cells, whereas ALLO responses can be generated by either CD4+ or CD4- T cells. Thus, the selective loss of S+X responses probably reflects the loss of CD4+ cells during AIDS development. Using the above approach, we have tested T cell functions in AIDS patients undergoing therapy with the thymidine analog AZT. Some patients undergoing AZT exhibited a restoration of T cell immune function, but this restoration of function has gradually decreased with time.

HTLV-III抗体（+）和 （ - ）供体的增生（3H）和细胞介导的供体 淋巴解（CML）到流感病毒（S+X）和HLA同种异体 细胞（Allo）。 在抗体（+）供体中，约50％ 未能回应S+X，而所有人都回应了Allo，除了 艾滋病关键阶段的患者对 免疫原的类型。 使用抗体（ - ）供体的PBL我们通过细胞证明 S+X T细胞响应的分馏技术有义务 使用CD4+ T细胞，而Allo响应可以由 CD4+或CD4-T细胞。 因此，S+X的选择性损失 响应可能反映了艾滋病期间CD4+细胞的损失 发展。 使用上述方法，我们已经测试了艾滋病中的T细胞功能 接受胸苷类似AZT治疗的患者。 一些接受AZT的患者表现出T细胞的修复 免疫功能，但是这种功能的恢复逐渐 随着时间的流逝减少。