BONE AND TOOTH GENE REGULATION

骨骼和牙齿基因调控

• 批准号: 3963734
• 负责人: M F YOUNG
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3963734
• 关键词: RNA; ameloblasts; antibody; antiserum; bone development; cell free system; complementary DNA; dentinogenesis; extracellular matrix; gene expression; genetic library; genetic regulation; human tissue; molecular cloning; tissue /cell culture; tooth; tooth enamel

The matrix proteins of bones and teeth play key roles in the structure and function of these tissues. Our objective in this investigation is to study the biosynthesis of these macromolecules and to understand the regulation of their expression. The expression of bone and enamel matrix proteins have been studied by constructing recombinant cDNA libraries from ameloblast and bone cell mRNA. cDNA clones encoding several bone and tooth matrix proteins were isolated using expressing DNA vectors and polyclonal antisera directed against individual bone matrix and ameloblast proteins. The clones were used to study the primary structure and regulation of expression of these genes in ameloblast tissue and in cultured bone cells.

骨骼和牙齿的基质蛋白在结构中起关键作用， 这些组织的功能。 我们在调查中的目标是研究 这些大分子的生物合成并了解调节 他们的表达。 骨和搪瓷基质蛋白的表达已经通过 constructing recombinant cDNA libraries from ameloblast and bone cell mRNA。 编码几个骨和牙齿基质蛋白的cDNA克隆是 使用表达的DNA载体和定向的多克隆抗血清分离 针对单个骨基质和成纤维细胞蛋白。 克隆是 用于研究这些表达的主要结构和调节 成成组织组织和培养的骨细胞中的基因。