STRUCTURE AND BONE MATRIX GENE EXPRESSION

结构和骨基质基因表达

• 批准号: 3917115
• 负责人: M F YOUNG
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3917115
• 关键词: RNA; antibody; antiserum; bone development; complementary DNA; extracellular matrix; gene expression; genetic library; genetic recombination; genetic regulation; human tissue; messenger RNA; molecular cloning; osteocytes; protein biosynthesis; protein structure; protein structure function; tissue /cell culture

The matrix protein of bones play key roles in the structure and function of these tissues. Our objective in this investigation is to study the biosynthesis of these macromolecules and to understand the regulation of their expression. The expression of bone matrix proteins have been studied by constructing recombinant cDNA libraries from bone cell mRNA. cDNA clones encoding several bone and tooth matrix proteins have been isolated using expression DNA vectors and polyclonal antisera directed against individual bone matrix and ameloblast proteins. The clones were used to study the primary structure and regulation of expression of these genes in cultured bone cells.

骨骼的基质蛋白在结构中起关键作用， 这些组织的功能。 我们在这项调查中的目标是 研究这些大分子的生物合成并理解 他们表达的调节。 骨基质蛋白的表达已通过 从骨细胞mRNA构建重组cDNA文库。 cDNA 编码多个骨和牙齿基质蛋白的克隆已经 使用表达DNA载体和多克隆抗血清分离 针对单个骨基质和成纤维细胞蛋白。 这些克隆用于研究主要结构和调节 这些基因在培养的骨细胞中的表达。