STRUCTURE AND BONE MATRIX GENE EXPRESSION

结构和骨基质基因表达

• 批准号: 3775660
• 负责人: M F YOUNG
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3775660
• 关键词: aging; ameloblasts; bone; bone development; complementary DNA; extracellular matrix proteins; gene expression; gene induction /repression; genetic library; genetic regulatory element; genetically modified animals; human genetic material tag; human tissue; laboratory mouse; messenger RNA; molecular cloning; protein biosynthesis; protein structure function; structural genes; tissue /cell culture; tooth

The matrix proteins of bones and teeth play key roles in the structure and function of these tissues. Our objective is to study the structure and function of these macromolecules and to understand the regulation of their expression. The structures of bone and tooth matrix proteins have been studied by constructing recombinant cDNA libraries from bone or ameloblast cell mRNA. cDNAs encoding several bone and tooth matrix proteins were isolated. The clones and antibodies were used to determine the primary structure and mode of expression of the genes in cultured cells and intact tissue. The corresponding genomic DNAs have also been isolated and used to determine the intron-exon organization of these genes and the elements that potentially regulate their expression during development. Studies are underway using transgenic mice to identify the function of the matrix proteins and the elements that regulate their expression during development and aging in vivo.

骨骼和牙齿的基质蛋白在结构中起关键作用 这些组织的功能。 我们的目标是研究结构 这些大分子的功能，并了解 他们的表达。 骨和牙基质蛋白的结构具有 通过从骨头构建重组cDNA库来研究 成熟细胞mRNA。 编码几个骨和牙齿矩阵的cDNA 分离蛋白质。 克隆和抗体用于确定 基因在培养的基因表达方式和表达方式 细胞和完整的组织。 相应的基因组DNA也已经 孤立并用于确定这些内含子外观组织 基因和可能调节其表达的元素 发展。 使用转基因小鼠正在进行研究，以识别 基质蛋白的功能及其调节的元素 在体内发育和衰老期间的表达。