PEPTIDE/PROTEIN ANALYSIS FACILITY

肽/蛋白质分析设备

• 批准号: 3521323
• 负责人: C RONALD KAHN
• 金额: $ 23.7万
• 依托单位: JOSLIN DIABETES CENTER
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-06-02 至 1992-06-01
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3521323
• 关键词: biomedical equipment purchase; biomedical equipment resource; biomedical facility; diabetes mellitus; insulin; peptide structure; protein purification; protein sequence

This shared instrument grant entitled "Peptide/protein analysis facility" is a request from the Joslin Diabetes Center for funds to purchase equipment to carry out protein and peptide composition and sequence analysis and purification. The Joslin is a combined research institute and patient care facility dedicated to the understanding and treatment of diabetes and related disorders. Research interests are broad, ranging from basic biochemical and molecular mechanisms, to the development of treatments for diabetes and its complications. In recent years, the scope of protein sequence analysis has changed from the aim of determining the complete primary structure of a protein by sequence analysis of peptides, to being an essential component towards an integrated protein data base, summarizing the structural, functional and clinical information from each separated protein. Many current and future NIH supported projects conducted by investigators at the Joslin as well as our close collaborators at other Harvard institutes have evolved to the point where protein purification and primary sequence analysis is absolutely necessary. A recurrent theme in many proposals emphasizes the need for a protein purification and sequencing facility which places top priority on projects focusing on diabetes related questions so that relevant cloning projects and structure/function studies can move forward in a timely way. The proposed "Peptide/protein analysis facility" will complement the recently established a peptide synthesis facility at the Joslin. All synthetic peptides from routine syntheses will be analyzed for composition, and products of more complex syntheses will be analyzed for composition, and products of more complex syntheses will be sequenced as well. Examples of additional projects in which protein sequence analyses are essential include: (1) the analysis of protein antigens on islet cells that are responsible for the development of diabetes; (2) mapping the 3-dimensional insulin binding pocket of the insulin receptor; (3) identification of serine, threonine and tyrosine phosphorylation sites in the insulin receptor and other proteins that are important for biological signal transmission and regulation. Moreover, the facility is essential for the identification, immunological analysis and cloning of (1) endopeptidases responsible for processing proinsulin, (2) substrates of the insulin and insulin-like growth factor I receptor kinase, (3) phosphotyrosine phosphatases which play a role in the regulation of insulin receptor signal transmission, (4) activin and inhibin receptors in the pituitary gland and (5) DNA binding proteins and transcription factors. These are examples of the projects currently underway that will utilize the protein analysis/sequencing facility at the Joslin Diabetes Center. We foresee a very substantial and increasing need for this facility as the Center continues to grow.

这种共享的仪器赠款标题为“肽/蛋白质分析设施” 是乔斯林糖尿病中心购买资金的要求 进行蛋白质和肽组成以及序列的设备 分析和纯化。 乔斯林是一个合并的研究所， 致力于理解和治疗的患者护理设施 糖尿病和相关疾病。 研究兴趣广泛，从 基本的生化和分子机制，发展 糖尿病及其并发症的治疗方法。 近年来，范围 蛋白质序列分析已从确定的目的变化 通过肽的序列分析，蛋白质的完整初级结构， 成为迈向综合蛋白质数据库的重要组成部分， 总结每个的结构，功能和临床信息 分离的蛋白质。 许多当前和未来的NIH支持项目 由乔斯林的调查员以及我们的密切合作者进行 在哈佛的其他机构已经演变为蛋白质 纯化和主要序列分析是绝对必要的。 一个 许多提案中的经常性主题强调了对蛋白质的需求 纯化和测序设施将重点放在项目上 专注于糖尿病相关的问题，以便相关的克隆项目 结构/功能研究可以及时进行。 提出的“肽/蛋白质分析设施”将补充 最近在乔斯林建立了肽合成设施。 全部 将分析来自常规合成的合成肽的组成，以获取组成， 将分析更复杂合成的产品的组成， 以及更复杂的合成产品的产品也将进行测序。 例子 在其他项目中，蛋白质序列分析是必不可少的 包括：（1）胰岛细胞上蛋白质抗原的分析 负责糖尿病的发展； （2）映射3维 胰岛素受体的胰岛素结合袋； （3）识别 胰岛素中的丝氨酸，苏氨酸和酪氨酸磷酸化位点 受体和其他对于生物信号很重要的蛋白质 传输和调节。 而且，该设施对于 （1）内肽酶的鉴定，免疫分析和克隆 负责处理促硫素，（2）胰岛素的底物和 胰岛素样生长因子I受体激酶，（3）磷酸酪氨酸 在调节胰岛素受体信号中起作用的磷酸酶 （4）垂体中的激活素和抑制素受体， （5）DNA结合蛋白和转录因子。 这些是目前正在进行的项目的示例 乔斯林糖尿病中心的蛋白质分析/测序设施。 我们 预见到对该设施的非常巨大的需求 中心继续增长。