MEMBRANE PROTEIN CRYSTALLIZATION WORKSHOP

膜蛋白结晶车间

• 批准号: 3434765
• 负责人: JANUSZ M SOWADSKI
• 金额: $ 1万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-01 至 1994-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3434765
• 关键词: crystallization; membrane proteins; workshop

Membrane bound proteins perform many important functions in biology:transport of nutrients, gated ion channels, receptors, neurotransmitters. Such proteins are the first recipients of signals entering the cell, and provide via phosphorylation or de-phosphorylation the bridge between cells and their environment. Progress in the area of membrane protein crystallization is essential for the acceleration of progress of structural studies of membrane proteins. The aim of this workshop is to disseminate the techniques used in the successful crystallization of membrane proteins in a hands-on fashion to the participants. The forum for the workshop is the Fifth International Conference for the Crystallization of Biological Macromolecules (ICCBM). This creates the unprecedented opportunity to integrate the crystallization of membrane proteins within the expert knowledge available at the ICCBM. The workshop avails itself of international experts in the field of membrane protein crystallization and of the leaders in theoretical and practical aspect of macromolecular crystal growth. The theoretical part of the workshop focuses on expression, solubilization and purification of membrane proteins for crystallization and covers in detail all aspects of detergents, exchange, CMC, mixed micelles, amphiphiles etc. The practical aspects deals with the unique crystallization methodology needed to handle membrane proteins compared to soluble proteins. The seminars include: Detergent structure in membrane protein crystals by neutron diffraction. General properties of membrane proteins. Role of detergents on the crystallization of membrane proteins and detergent packing in membrane protein crystals. Crystallization of membrane proteins for three-dimensional X-ray crystallography. Two-dimensional crystallization of membrane proteins.

膜结合蛋白在以下方面发挥许多重要功能： 生物学：营养物质的运输、门控离子通道、受体、 神经递质。 这些蛋白质是信号的第一个接收者 进入细胞，并通过磷酸化或去磷酸化提供 细胞与其环境之间的桥梁。 该领域的进展 膜蛋白结晶对于加速 膜蛋白结构研究进展. 本次研讨会的目的是传播所使用的技术 以动手方式成功结晶膜蛋白 参与者。 研讨会的论坛是第五届国际 生物大分子结晶会议（ICCBM）。 这创造了前所未有的机会来整合 在专业知识范围内膜蛋白的结晶 可在 ICCBM 获取。 本次研讨会充分利用国际 膜蛋白结晶领域的专家 高分子晶体理论和实践方面的领导者 生长。 研讨会的理论部分侧重于表达， 膜蛋白的溶解和纯化以进行结晶 并详细涵盖了洗涤剂、交换、CMC、混合的各个方面 胶束、两亲物等。实际方面涉及独特的 处理膜蛋白所需的结晶方法比较 到可溶性蛋白质。 研讨会包括： 通过中子衍射观察膜蛋白晶体中的洗涤剂结构。 膜蛋白的一般特性。 洗涤剂的作用 膜蛋白的结晶和膜中洗涤剂的堆积 蛋白质晶体。 三维 X 射线膜蛋白结晶 晶体学。 膜蛋白的二维结晶。