A Synchrotron Radiation Structural Biology Resource

同步辐射结构生物学资源

• 批准号: 10350697
• 负责人: Aina E. Cohen
• 金额: $ 168.78万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-01 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10350697
• 关键词: Acute; Automatic Data Processing; Automation; Biomedical Research; Clinic; Collaborations; Collection; Communities; Complex; Computer software; Coupled; Cryoelectron Microscopy; Cryopreservation; Crystallization; Crystallography; Custom; Data; Data Analyses; Data Collection; Data Set; Dehydration; Discipline; Documentation; Education and Outreach; Educational workshop; Fostering; Freezing; Generations; Goals; Humidity; Ice; In Situ; Individual; Instruction; Internet; Investigation; Location; Manuals; Measurement; Membrane Proteins; Methodology; Methods; Mind; Molecular Biology; Monitor; Multiprotein Complexes; Peer Review; Performance; Process; Protein Dynamics; Publications; Radiation; Raman Spectrum Analysis; Reaction; Recording of previous events; Research; Resolution; Resources; Roentgen Rays; Running; Sampling; Schools; Science; Services; Site; Source; Structure; Synchrotrons; System; Techniques; Technology; Time; Training; Training Programs; Training Support; Vision; absorption; base; beamline; biological systems; computerized data processing; data structure; design; detector; experience; experimental study; flexibility; innovation; instrumentation; lectures; metalloenzyme; novel; online resource; operation; outreach; outreach program; programs; protein complex; software development; spectroscopic imaging; structural biology; success; synchrotron radiation; temperature jump; tool; user-friendly; web portal; x-ray free-electron laser

Synchrotron radiation (SR) is an extremely bright and tunable x-ray source that enables forefront research in structural molecular biology (SMB). The goal of “A Synchrotron Structural Biology Resource" at the Stanford Synchrotron Radiation Lightsource (SSRL) is to provide users access to an integrated, mature SMB Resource with state-of-the-are synchrotron beam line facilities for macromolecular crystallography, x-ray absorption/emission/imaging spectroscopy and small angle x-ray scattering/diffraction, that form the three Resource Technical Operations Cores. Special emphasis is to create streamlined user access, provide dedicated user support and services, to train users, and to perform outreach on the scientific benefits of the capabilities of these specialized, yet mature facilities to the biomedical research community. Multiple innovative access mechanisms will be provided, that will include both single- and multiple-technique approaches to access the Cores, based on a transparent and robust external peer-review process. A multifaceted training and outreach program will include individual training, workshops, summer schools, on-the-road, and support clinic segments by experienced science and technical staff. A Resource web portal will provide streamlined scientific, technical, training, outreach and administrative material and other relevant information. A principal aim is to optimize and maintain, for reliable and forefront performance, experimental facilities and instrumentation, detectors, software and computing and network capabilities on the SMB Resource’s 8+ beam lines at SSRL, capitalizing on the high SR x-ray performance of SSRL’s 3rd generation storage ring SPEAR3, and at the MFX station at LCLS. A multi-facility proposal mechanism will facilitate access to complementary Cryo-EM and LCLS stations on-site at SLAC. With a specialized focus on user training, support and service, the SMB Resource will enable the scientific success for challenging and routine projects in the biomedical sciences.

同步辐射 (SR) 是一种极其明亮且可调谐的 X 射线源，可实现前沿 结构分子生物学（SMB）的研究“同步加速器结构生物学”的目标。 斯坦福同步辐射光源 (SSRL) 的资源”旨在为用户提供访问 集成、成熟的 SMB 资源，具有最先进的同步加速器光束线设施，用于 大分子晶体学、X射线吸收/发射/成像光谱和小分子晶体学 角 X 射线散射/衍射，形成三个资源技术运营核心。 特别强调的是创建简化的用户访问、提供专门的用户支持和 服务，培训用户，并宣传其能力的科学效益 这些专业而成熟的设施为生物医学研究界带来了多重创新。 将提供访问机制，其中包括单一技术和多重技术 基于透明和强大的外部同行评审的访问核心的方法 多方面的培训和外展计划将包括个人培训、研讨会、 由经验丰富的科学和技术人员提供的暑期学校、路上培训和支持诊所部分 资源门户网站将提供简化的科学、技术、培训、外展和服务。 管理材料和其他相关信息的主要目的是优化和维护， 提供可靠和前沿的性能、实验设施和仪器、探测器、 SSRL SMB 资源的 8 条以上光束线上的软件、计算和网络功能， 利用 SSRL 第三代存储环 SPEAR3 的高 SR X 射线性能， 在 LCLS 的 MFX 站，多设施提案机制将促进访问。 SLAC 现场有互补的 Cryo-EM 和 LCLS 站，专门关注用户。 培训、支持和服务，SMB 资源将使挑战的科学成功 和生物医学科学的常规项目。