PHYSICAL CHEMISTRY OF BIOLOGICAL MACROMOLECULES

生物大分子物理化学

• 批准号: 3789389
• 负责人: M S LEWIS
• 金额: --
• 依托单位: NATIONAL CENTER FOR RESEARCH RESOURCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3789389
• 关键词: RNA directed DNA polymerase; acidity /alkalinity; amyloid proteins; analytical ultracentrifugation; biophysics; calcium flux; chemical structure function; human immunodeficiency virus 1; human tissue; intermolecular interaction; macromolecule; mathematical model; molecular shape; molecular size; nucleocapsid; protein structure function; secretory protein; stress proteins; thermodynamics; virus RNA

The purpose of this project is to study the physical properties of a wide variety of biological macromolecules, with the goal of correlating these properties to the structure and function of the macromolecules. The emphasis is on the thermodynamics of the interactions of these macromolecules and on their molecular size and shape. Analytical ultracentrifugation and mathematical modeling are the principal research techniques used. Studies on HIV reverse transcriptase have been directed toward investigating the thermodynamics of the homogeneous association of the P66 subunit, and the heterogeneous association of P66 with the P51 subunit. Studies on chromagranin A have been directed toward determining the domain of this protein responsible for aggregation, and investigating the effects of pH and calcium ion concentration on the thermodynamics of the aggregative properties of a peptide encompassing this domain. Studies on CEB-63 and CEB-35, leucine-zipper peptides, have been directed toward determining the thermodynamic parameters characterizing their reversible aggregation. Studies on heat shock proteins and nucleic acids have been directed toward investigating the nature of their interactions. Studies on the interaction of HIV-1 RNA with nucleocapsid protein have been directed toward a more quantitative understanding of this interaction. Studies on the beta amyloid peptide have been directed toward understanding the aggregative properties of this peptide and its possible relationship to Alzheimer's disease.

该项目的目的是研究广泛的物理特性 各种生物大分子，其目的是将其关联 大分子的结构和功能的性质。 这 重点是这些相互作用的热力学 大分子及其分子大小和形状。 分析 超速离心和数学建模是主要研究 使用的技术。 HIV逆转录酶的研究已针对 调查均质关联的热力学 p66亚基和p66与p51的异质关联 亚基。 关于Chromagranin A的研究已针对确定 该蛋白质的领域负责聚集并研究 pH和钙离子浓度对热力学的影响 包含该域的肽的聚合特性。 有关CEB-63和CEB-35，亮氨酸Zipper肽的研究已被定向 要确定表征其热力学参数 可逆聚集。 热激蛋白和核酸的研究 已致力于调查其相互作用的性质。 HIV-1 RNA与核蛋白蛋白的相互作用的研究 是针对对此有更定量理解的 相互作用。 有关β-淀粉样肽的研究已被定向 要了解该肽及其的总体特性 可能与阿尔茨海默氏病的关系。