PHYSICAL CHEMISTRY OF BIOLOGICAL MACROMOLECULES

生物大分子物理化学

• 批准号: 4705611
• 负责人: M S LEWIS
• 金额: --
• 依托单位: DIVISION OF RESEARCH SERVICES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4705611
• 关键词: analytical ultracentrifugation; antifibrinolytic agents; chemical chain length; chemical structure function; fibrinogen; hydropathy; macromolecule; mathematical model; molecular biology; molecular shape; molecular size; molecular weight; plasminogen; polymers; retina; retinaldehyde; thermodynamics; visual photoreceptor

The purpose of this project is to study the physical properties of a wide variety of biological macromolecules with the goal of correlating these properties to the structure and function of the macromolecules. The emphasis is on the thermodynamics of the interactions of these macromolecules and on their molecular size and shape. Analytical ultracentrifugation and mathematical modeling are the principal research techniques used. Studies on the association of fibrinogen with other proteins involved in blood clotting and fibrinolysis have been continued. Studies currently in progess in this area deal with the association of fibrinogen with plasma and platelet Factor XIII and with the association of plasminogen with the D and E fragments of fibrinogen. Studies on the association of the A and B chains of reduced ricin indicate that the formation of the AB cmplex is reversible and that the temperature dependence of this reaction indicates that it appears to be entropically driven, suggesting that there is a strong hydrophobic interaction between A and B chains even in the absence of the disulfide bond. Cell toxicity studies indicate that reduced ricin is more toxic than native ricin at high concentrations, indicating that the disulfide bond between the chains is not necessary for biologcial activity. The small and large forms of heparin sulfate proteoglycan isolated from EHS tumor basement membrane have been studied by electron microscopy and by analytical ultracentrifugation. These studies indicate that these proteoglycans show similarities in the number and size of the heparin sulfate side chains but differ in the length of the protein core.

该项目的目的是研究广泛的物理特性 将这些生物大分子与将其关联的目的多种多样 大分子的结构和功能的性质。 这 重点是这些相互作用的热力学 大分子及其分子大小和形状。 分析 超速离心和数学建模是主要研究 使用的技术。 研究纤维蛋白原与其他参与的蛋白质的关联 血液凝结和纤维蛋白溶解持续。 目前正在研究的研究 该区域的进度涉及纤维蛋白原与血浆的关联 和血小板因子XIII以及纤溶酶原与D的关联 和纤维蛋白原的E片段。 关于ricin降低的A和B链的关联的研究表明 AB CMPLEX的形成是可逆的，温度 该反应的依赖性表明它似乎是熵的 驱动，表明在 即使在没有二硫键的情况下，B链也是如此。 细胞毒性 研究表明，减少的利辛蛋白比在高的天然利辛蛋白更具毒性 浓度，表明链之间的二硫键是 生物学活性没有必要。 从EHS分离的小和大形式的硫酸肝素蛋白聚糖 肿瘤基底膜已经通过电子显微镜和 分析性超速离心。 这些研究表明这些 蛋白聚糖在肝素的数量和大小上显示出相似之处 硫酸盐侧链，但蛋白质核的长度有所不同。