PHYSICAL CHEMISTRY OF BIOLOGICAL MACROMOLECULES

生物大分子物理化学

• 批准号: 3896167
• 负责人: M S LEWIS
• 金额: --
• 依托单位: NATIONAL CENTER FOR RESEARCH RESOURCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3896167
• 关键词: actins; analytical ultracentrifugation; arginase; cytokine receptors; enzyme mechanism; enzyme structure; interleukin 2; keratin; macromolecule; mathematical model; microtubules; molecular biology; molecular size; molecular weight; nucleotides; protein glutamine gamma glutamyltransferase; protein structure function; tubulin

The purpose of this project is to study the physical properties of a wide variety of biological macromolecules with the goal of correlating these properties to the structure and function of the macromolecules. The emphasis is on the thermodynamics of the interactions of these macromolecules and on their molecular size and shape. Analytical ultracentrifugation and mathematical modeling are the principal research techniques used. Studies on actin have been directed toward investigating the mechanisms involved in actin polymerization and the interaction of actin with actobindin. Studies on epidermal transglutaminase have involved characterization of the proenzyme form and study of the mechanism of activation. Studies on yeast arginase have been directed toward studying the reversible homogeneous association of this enzyme and the role of metal ions in controlling this. Studies of various deoxynucleotides have been directed toward investigating the reversible monomer-dimer equilibrium as a function of structure. Studies on alpha-helical fragments of keratin have been directed toward elucidating their role in keratin filaments. Studies on interleukin-2 receptors have been directed toward an elucidiction of the structure of the molecule. Studies on trypanosomatid proteins and tubulin and microtubules have been directed to an understanding of their interaction.

该项目的目的是研究广泛的物理特性 将这些生物大分子与将其关联的目的多种多样 大分子的结构和功能的性质。这 重点是这些相互作用的热力学 大分子及其分子大小和形状。分析 超速离心和数学建模是主要研究 使用的技术。 肌动蛋白的研究已针对研究机制 参与肌动蛋白聚合以及肌动蛋白与 Actobindin。表皮转谷氨酰胺酶的研究涉及 原始酶形式的表征和研究 激活。酵母精氨酸酶的研究已针对研究 该酶的可逆均匀关联和金属的作用 离子控制了这一点。各种脱氧核苷酸的研究已经 致力于研究可逆的单体二聚体平衡 结构的功能。关于角蛋白α-螺旋片段的研究 致力于阐明其在角蛋白丝中的作用。研究 在白介素-2受体上已针对阐明 分子的结构。关于锥形蛋白和微管蛋白的研究 和微管已针对他们的理解 相互作用。