Human Tissues, Lipidomics, and Proteomics Core

人体组织、脂质组学和蛋白质组学核心

基本信息

批准号：
10628988
负责人：
Jose Orlando Aleman
金额：
$ 23.97万
依托单位：
NYU LONG ISLAND SCHOOL OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-05-01 至 2028-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10628988
关键词：
Adipocytes Adipose tissue Apolipoproteins Apolipoproteins B Arterial Fatty Streak Atherosclerosis Basic Science Biogenesis Bioinformatics Biological Assay Biology Body Weight decreased Cardiovascular Diseases Cardiovascular system Catabolism Centrifugation Ceramides Clinical Communities Complement Data Data Set Diabetes Mellitus Family Fasting Gastrectomy Goals Health Human Hypertriglyceridemia Individual Institution Laboratories Lasers Lipids Lipoproteins Liver Macrophage Mass Spectrum Analysis Metabolism Methods Molecular Molecular Sieve Chromatography Mus Obesity Outcome Plasma Procedures Process Protein Family Proteins Proteomics Regulation Research Research Personnel Risk Risk Marker Risk Reduction Role Sampling Services Standardization Systems Biology Technology Tissues Translating Triglycerides Ultracentrifugation analytical method analytical tool bioinformatics tool cardiovascular disorder risk clinical application clinical practice clinical risk clinically relevant cohort cost effectiveness extracellular vesicles human tissue instrumentation interest lipidomics metabolomics mouse model novel particle phenotypic data pre-clinical

Adipocytes Adipose tissue Apolipoproteins Apolipoproteins B Arterial Fatty Streak Atherosclerosis Basic Science Biogenesis Bioinformatics Biological Assay Biology Body Weight decreased Cardiovascular Diseases Cardiovascular system Catabolism Centrifugation Ceramides Clinical Communities Complement Data Data Set Diabetes Mellitus Family Fasting Gastrectomy Goals Health Human Hypertriglyceridemia Individual Institution Laboratories Lasers Lipids Lipoproteins Liver Macrophage Mass Spectrum Analysis Metabolism Methods Molecular Molecular Sieve Chromatography Mus Obesity Outcome Plasma Procedures Process Protein Family Proteins Proteomics Regulation Research Research Personnel Risk Risk Marker Risk Reduction Role Sampling Services Standardization Systems Biology Technology Tissues Translating Triglycerides Ultracentrifugation analytical method analytical tool bioinformatics tool cardiovascular disorder risk clinical application clinical practice clinical risk clinically relevant cohort cost effectiveness extracellular vesicles human tissue instrumentation interest lipidomics metabolomics mouse model novel particle phenotypic data pre-clinical

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项目摘要

SUMMARY – HUMAN TISSUES, LIPOPROTEINS, LIPIDOMICS, & PROTEOMICS CORE (C2) The Human Tissues, Lipoproteins, Lipidomics, and Proteomics Core (Core 2, C2) will focus on providing human tissues and analytical capabilities to all three projects to enhance translatability of mouse studies proposed in P1–P3 to clinical practice in humans. In parallel, Core 2 will provide lipoprotein, lipidomic, and proteomic analyses currently not provided by our institutional Metabolomics Core. The cardiovascular research community has shown many important correlations between clinical outcomes and the plasma levels of atherogenic particles of different densities, as determined by ultracentrifugation. More detailed analyses are now uncovering additional risk for cardiovascular disease (CVD) within these particles, such as the content of apoC3 and ceramides. For this PPG, Core 2 will provide a more granular analysis of lipoproteins isolated by centrifugation and size exclusion chromatography through both directed and unbiased lipidomic and proteomic analyses of these particles. In addition, clinical samples (sera, liver, adipose) obtained by the core director will be provided to investigators in all three projects. These services will avoid the need for PPG investigators to maintain the required instrumentation in their own laboratories or use expensive commercial services. By centralizing and standardizing procedures, Core 2 provides a common set of analytical tools to provide a unified understanding of molecular mechanisms involved in pathophysiologic processes of atherosclerosis, macrophage biology, regulation of triglyceride-rich lipoproteins, and obesity. By complementing and integrating with the service provided by Core 1, Core 2 staff also provide bioinformatics support to analyze and interpret proteomic and lipidomic data sets, making Core 2 capabilities available to PPG investigators with little background or expertise in these analytical technologies. A key long-term goal is to integrate quantitative analyses of lipidomic data with functional studies to provide a systems biology view of diabetes-related cardiovascular disease. Core 2 will also develop new analytical methods tailored to specific research objectives of PPG investigators. Specifically, we will obtain and use standards to quantify and discover candidate lipids whose role in atherosclerosis is being investigated. These methods will be extensively used by PPG investigators. Core 2 will offer these services with optimal efficiency and cost-effectiveness, avoiding the need for individuals to pursue such analyses outside the PPG.

摘要 - 人体组织，脂蛋白，脂质组学和蛋白质组学核心（C2）人体组织，脂蛋白，脂肪组学和蛋白质组学核心（Core 2，C2）将重点放在为所有三个项目提供人体组织和分析能力，以增强可翻译性在P1 – P3中提出的小鼠研究对人类的临床实践。同时，核心2将提供脂蛋白，脂质组和蛋白质组学分析目前未提供我们的机构代谢组学核心。心血管研究社区显示了许多重要的临床结局与血浆的血浆水平之间的相关性通过超速离心确定的密度不同。现在进行了更详细的分析发现这些颗粒中心血管疾病（CVD）的额外风险，例如 ApoC3和神经酰胺的含量。对于此PPG，Core 2将提供更详细的分析通过离心和大小排除色谱分离的脂蛋白通过两种定向以及这些颗粒的无偏脂肪组和蛋白质组学分析。另外，临床样品（血清，肝脏，脂肪）将由核心主任获得的所有三个项目。这些服务将避免PPG调查人员维护所需的需求仪器在自己的实验室中或使用昂贵的商业服务。通过集中化和标准化程序，Core 2提供了一组共同的分析工具，以提供对参与病理生理过程的分子机制的统一理解动脉粥样硬化，巨噬细胞生物学，富含甘油三酸酯的脂蛋白的调节和肥胖。经过核心2员工还与Core 1提供的服务补充并集成生物信息学支持以分析和解释蛋白质组学和脂肪组数据集，使核心 PPG调查人员可使用的2功能，这些功能很少或专业知识分析技术。一个关键的长期目标是整合脂质组的定量分析具有功能研究的数据，以提供与糖尿病相关心血管的系统生物学视图疾病。核心2还将开发针对特定研究目标量身定制的新分析方法 PPG调查人员。具体来说，我们将获得并使用标准来量化和发现正在研究其在动脉粥样硬化中作用的候选脂质。这些方法将是 PPG研究人员广泛使用。 Core 2将以最佳效率提供这些服务和成本效益，避免个人需要在以外进行此类分析 ppg。