ForceBiology

原力生物学

• 批准号: EP/Y033000/1
• 负责人: Nikolaj Gadegaard
• 金额: $ 16.19万
• 依托单位: University of Glasgow
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2024
• 资助国家: 英国
• 起止时间: 2024 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FY033000%2F1
• 关键词: ForceBiology

Mechanical cues play crucial role in cell function such as migration, proliferation, invasion and contraction. Cellular forces can be used for discovering and assessing the effects of new drugs but has been left largely unexplored, mainly due to lack of HTS and quantitative assays. Despite knowledge of their significance in disease, drug developers have had limited access to force-generating assays, especially in the context of high-throughput screening. Presently there are few in vitro platforms available on the market for measuring migration and contractility on a platform with reduced cost and complexity. Therefore, there is pressing need to develop and explore cellular forces to investigate, screen and discover new drug compounds in high throughput manner. Research has shown that mechanical cues and contractility can dictate the fate of a human cell. In cardiovascular disease (CVD), mechanical cues give rises to stiffening of the cellular microenvironment causing life threatening conditions such as hypertension, and hyperlipidemia. According to WHO, 4 out of 5 CVD deaths, can be attributable to heart attacks and strokes. Vascular smooth muscle cells (VSMCs) comprise the mechanically active component of the blood vessel wall endowing it with the ability to constrict and dilate. To evaluate the viability of a muscle tissue, it is essential to measure the tissue's contractile performance as well as to control its structure. Accurate contractility data can aid in development of more effective and safer drugs. the processes of contractile force generation and transmission play an important function role in defining the mechanical efficiency of VSMCs. Today there are no tools on the market to quantify the contractile forces in VSMCs in a reliable, high-content platform suitable for the pharma industry. ForceBiology addresses this market need.

机械提示在细胞功能中起着至关重要的作用，例如迁移，增殖，入侵和收缩。细胞力可用于发现和评估新药的作用，但在很大程度上没有探索，这主要是由于缺乏HTS和定量测定。尽管知识对疾病的重要性知识，但药物开发人员的使用有限，尤其是在高通量筛查的背景下。目前，市场上很少有体外平台可用于衡量成本和复杂性降低的平台上的迁移和收缩力。因此，有必要开发和探索细胞力以调查，筛选和发现高通量方式的新药化合物。研究表明，机械提示和收缩力可以决定人类细胞的命运。在心血管疾病（CVD）中，机械提示使细胞微环境的僵硬增加，从而导致威胁生命的疾病，例如高血压和高脂血症。根据谁的说法，在5例CVD死亡中，有4例可以归因于心脏病发作和中风。血管平滑肌细胞（VSMC）构成了血管壁的机械活性成分，使其具有收缩和扩张的能力。为了评估肌肉组织的生存能力，必须测量组织的收缩性能以及控制其结构。准确的收缩性数据可以帮助开发更有效，更安全的药物。收缩力的产生和传输过程在定义VSMC的机械效率方面起着重要功能。如今，市场上没有工具可以在适合制药行业的可靠，高内心平台中量化VSMC中的收缩力。 Forcebiology解决了这个市场需求。