PURCHASE OF AN ANLYTICAL ULTRACENTRIFUGE

购买分析超速离心机

• 批准号: 2284194
• 负责人: Edward Eisenstein
• 金额: $ 13.7万
• 依托单位: UNIV OF MARYLAND, COLLEGE PARK
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-05-18 至 1995-05-17
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2284194
• 关键词: analytical ultracentrifugation; biomedical equipment purchase; structural biology

A major users group of nine structural biologists from the three campuses in the University of Maryland System seeks funding for an analytical ultracentrifuge to replace our 40 year old Model E ultracentrifuge at CARB. The major users group consists of Drs. Armstrong and Julin from College Park, Drs. Beckett and Pollack from Baltimore County, and Drs. Bryan, Eisenstein (PI), Mariuzza, McKenney and Polijak from CARB. This analytical ultracentrifuge will be used in our ongoing studies that are focussed on the determination of the quaternary structures of complex soluble and membrane associated proteins, the evaluation of the energetics of self- and heterologous-associations of proteins, the estimation of the strength of DNA-protein interactions, and the measurement of conformation changes promoted by small molecule binding to their macromolecular targets. The newly designed state-of-the-art Optima XL-A analytical ultracentrifuge that we are requesting, which offers a new level of precision and sensitivity in detecting the distribution of a solute under the influence of a centrifugal field, would considerably expand the scope of problems we could address by overcoming many of the limitations of our now obsolete Model E. The most vastly improved features of the Optima XL-A relative to the Model E include stable rotor drives, accurate and precise temperature control, powerful yet user-friendly system software enabling complete computer control over run conditions and data collection, and a vacuum enclosed monochromator that provides the capability to detect nanogram quantities of proteins by scanning absorbance down to 190 nm. Furthermore, the acquisition of a new analytical ultracentrifuge is essential due to the frequent equipment failure associated with keeping a 40 year old instrument running, and because Beckman will no longer service our Model E after June 1994.

来自三个校园的九位结构生物学家的主要用户小组 在马里兰州大学，寻求用于分析的资金 超速离心替换我们40岁的型号E超离心机 碳水化合物。 主要用户组由DRS组成。阿姆斯特朗和朱琳 大学公园，博士。 来自巴尔的摩县的贝克特和波拉克和博士。 来自Carb的Bryan，Eisenstein（PI），Mariuzza，McKenney和Polijak。 这 分析性超级离心机将在我们正在进行的研究中使用 专注于确定复杂的第四纪结构 可溶性和膜相关蛋白，评估 蛋白质的自我和异源缔合的能量学， 估计DNA蛋白相互作用的强度和 小分子结合促进构象变化的测量 到它们的大分子靶标。 新设计的最先进的 我们要求的Optima XL-A分析超级离心机 在检测到 在离心场的影响下溶质的分布， 将大大扩大我们可以解决的问题的范围 克服我们现在过时的模型E的许多局限 相对于模型E，Optima XL-A的特征大大提高了 包括稳定的转子驱动器，准确而精确的温度控制， 功能强大但用户友好的系统软件可实现完整的计算机 控制运行条件和数据收集，并封闭真空 单色器提供了检测纳米图数量的能力 通过扫描吸光度降低至190 nm，蛋白质的蛋白质。 此外， 获得新的分析超速离心犯是必不可少的 与保持40岁有关的频繁设备故障 仪器运行，并且因为贝克曼将不再为我们的模型服务 E 1994年6月之后。