ELEMENTARY STEPS OF CHAPERONIN PROMOTED PROTEIN FOLDING

伴侣蛋白促进蛋白质折叠的基本步骤

基本信息

批准号：
2701592
负责人：
Edward Eisenstein
金额：
$ 17.22万
依托单位：
UNIVERSITY OF MD BIOTECHNOLOGY INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
1993
资助国家：
美国
起止时间：
1993-05-01 至 2000-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2701592
关键词：
X ray crystallography adenosine diphosphate adenosine triphosphate chemical binding chemical kinetics conformation molecular chaperones protein folding site directed mutagenesis

X ray crystallography adenosine diphosphate adenosine triphosphate chemical binding chemical kinetics conformation molecular chaperones protein folding site directed mutagenesis

项目摘要

DESCRIPTION: This is an application to continue support in years 4-8 for studies aimed at defining the mechanisms by which the molecular chaperone, GroEL, can assist the folding of denatured proteins. All of the research is to elucidate elementary steps of a single cycle of chaperonin facilitated protein folding. Many different experimental techniques are proposed ranging from site directed mutagenesis to X-ray crystallography and neutron scattering. The use of surface plasmon resonance is well used in these experiments. The proposed research would test 3 specific aims: 1) evaluate predictions of a model for GroEL function based on nucleotide promoted release of polypeptide chains. For example, the use of single-ring variants of GroEL would test the prediction that the ring with bound polypeptide binds nucleotides weakly, but promotes rapid non-native chain release. Further, it will be determined whether full nucleotide occupancy of a single ring is required for release of non-native chains. Methods would include hydrodynamic and scattering studies to determine nucleotide promoted conformational changes and whether they are concerted and follow saturation with nucleotide.

描述：这是在4 - 8年中继续支持的申请旨在定义分子的机制的研究伴侣，凹槽可以帮助折叠变性蛋白。所有人该研究是为了阐明单个周期的基本步骤伴侣蛋白促进蛋白质折叠。许多不同的实验提出的技术范围从定向诱变到X射线晶体学和中子散射。表面等离子体的使用共振在这些实验中很好地使用了。拟议的研究将测试3个特定目的：1）评估凹槽模型的预测基于核苷酸的功能促进了多肽链的释放。例如，使用凹槽的单环变体将测试预测具有结合多肽的环会弱结合核苷酸，但促进了快速的非本地链释放。此外，它将是确定单个环的全核苷酸占用率是否为释放非本地链的必需品。方法包括流体动力学和散射研究以确定核苷酸促进构象变化以及它们是否协同并遵循用核苷酸饱和。