CHOLECYSTOKININ & OPIOID INTERACTIONS

胆囊收缩素

• 批准号: 3068703
• 负责人: PATRICIA L FARIS
• 金额: $ 6.62万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-04-01 至 1990-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3068703
• 关键词: analgesia; appetite regulatory center; autoradiography; brain mapping; cholecystokinin; dorsal column; dynorphins; eating; electron microscopy; endorphins; enkephalins; histochemistry /cytochemistry; hormone inhibitor; hypothalamus; immunochemistry; laboratory rat; neuroendocrine system; neuropeptide receptor; neuropeptides; nutrition related tag; pain

In recent studies the applicant has demonstrated that cholecystokinin (CCK) antagonizes opiate-specific analgesia. Several lines of evidence suggest that CCK has effects opposite from those of opiates on other functions such as food intake. In this application for a level I RSDA, I propose to study the neuroanatomical organization of CCK and opiate systems in specific brain regions concerned with nociception and food intake and will seek evidence for neuroanatomical contacts between CCK and opiate systems through which modulatory effects of one system upon the other might occur. In addition, by biochemical, pharmacological and behavioral approaches I will seek further evidence for an interactive relationship between CCK and opiate systems in the regulation of nociception and food intake. My prior training has been primarily in behavioral and pharmacological methods. The research proposed herein will provide supervised training in a wide range of additional techniques (light and electron microscopic immunohistochemistry, receptor ligand autoradiography, retrograde and anterograde transmitter pathway tracing, selective neurochemical lesioning, radioimmunoassay, high performance liquid chromatography and receptor binding assays).

在最近的研究中，申请人表明胆囊动蛋白（CCK） 拮抗鸦片特异性镇痛。 几条证据表明 CCK的影响与阿片类药物对其他功能的影响相反 作为食物摄入量。 在此级别的I级i rsda中，我建议学习 CCK和鸦片系统的神经解剖组织特定 与伤害感受和食物摄入有关的大脑区域，并将寻求 CCK和阿片类系统之间神经解剖接触的证据 通过其中，一个系统对另一个系统的调节作用可能发生。 另外，通过生化，药理和行为方法I 将寻求进一步的证据证明CCK与 阿片类药物在调节伤害感受和食物摄入量中。 我的先验 培训主要是行为和药理方法。 这 本文提出的研究将提供广泛的监督培训 其他技术（光和电子显微镜 免疫组织化学，受体配体放射自显影，逆行和 顺行发送器途径跟踪，选择性神经化学病变， 放射免疫分子，高性能液相色谱和受体 绑定测定）。

项目成果

Antagonism of morphine analgesia by nonopioid cold-water swim analgesia: direct evidence for collateral inhibition.

非阿片类冷水游泳镇痛对吗啡镇痛的拮抗作用：附带抑制的直接证据。

• DOI: 10.1016/s0149-7634(05)80155-1
• 发表时间: 1990
• 期刊: Neuroscience and biobehavioral reviews
• 影响因子: 8.2
• 作者: Steinman,JL;Faris,PL;Mann,PE;Olney,JW;Komisaruk,BR;Willis,WD;Bodnar,RJ
• 通讯作者: Bodnar,RJ

Immunohistochemical localization of corticotropin-releasing factor in selected brain areas of the European starling (Sturnus vulgaris) and the song sparrow (Melospiza melodia).

欧洲椋鸟 (Sturnus vulgaris) 和歌麻雀 (Melospiza melodia) 选定脑区促肾上腺皮质激素释放因子的免疫组织化学定位。

• DOI: 10.1007/bf00221646
• 发表时间: 1989
• 期刊: Cell and tissue research
• 影响因子: 3.6
• 作者: Ball,GF;Faris,PL;Wingfield,JC
• 通讯作者: Wingfield,JC