CHOLECYSTOKININ & OPIOID INTERACTIONS

胆囊收缩素

• 批准号: 3068703
• 负责人: PATRICIA L FARIS
• 金额: $ 6.62万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-04-01 至 1990-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3068703
• 关键词: analgesia; appetite regulatory center; autoradiography; brain mapping; cholecystokinin; dorsal column; dynorphins; eating; electron microscopy; endorphins; enkephalins; histochemistry /cytochemistry; hormone inhibitor; hypothalamus; immunochemistry; laboratory rat; neuroendocrine system; neuropeptide receptor; neuropeptides; nutrition related tag; pain

In recent studies the applicant has demonstrated that cholecystokinin (CCK) antagonizes opiate-specific analgesia. Several lines of evidence suggest that CCK has effects opposite from those of opiates on other functions such as food intake. In this application for a level I RSDA, I propose to study the neuroanatomical organization of CCK and opiate systems in specific brain regions concerned with nociception and food intake and will seek evidence for neuroanatomical contacts between CCK and opiate systems through which modulatory effects of one system upon the other might occur. In addition, by biochemical, pharmacological and behavioral approaches I will seek further evidence for an interactive relationship between CCK and opiate systems in the regulation of nociception and food intake. My prior training has been primarily in behavioral and pharmacological methods. The research proposed herein will provide supervised training in a wide range of additional techniques (light and electron microscopic immunohistochemistry, receptor ligand autoradiography, retrograde and anterograde transmitter pathway tracing, selective neurochemical lesioning, radioimmunoassay, high performance liquid chromatography and receptor binding assays).

在最近的研究中，申请人已经证明胆囊收缩素（CCK） 拮抗阿片特异性镇痛作用。 几条证据表明 CCK 对其他功能的影响与阿片类药物相反，例如 作为食物摄入量。 在本次 I 级 RSDA 申请中，我建议学习 CCK 和阿片系统的神经解剖学组织 与伤害感受和食物摄入有关的大脑区域会寻求 CCK 和阿片系统之间神经解剖学联系的证据 通过这种方式，一个系统可能对另一个系统产生调节作用。 此外，通过生化、药理学和行为方法我 将寻求进一步的证据来证明 CCK 和 阿片系统调节伤害感受和食物摄入。 我之前的 培训主要是行为和药理学方法。 这 本文提出的研究将提供广泛的监督培训 附加技术（光学和电子显微镜 免疫组织化学、受体配体放射自显影、逆行和 顺行递质通路追踪、选择性神经化学损伤、 放射免疫分析、高效液相色谱和受体 结合测定）。

项目成果

Antagonism of morphine analgesia by nonopioid cold-water swim analgesia: direct evidence for collateral inhibition.

非阿片类冷水游泳镇痛对吗啡镇痛的拮抗作用：附带抑制的直接证据。

• DOI: 10.1016/s0149-7634(05)80155-1
• 发表时间: 1990
• 期刊: Neuroscience and biobehavioral reviews
• 影响因子: 8.2
• 作者: Steinman,JL;Faris,PL;Mann,PE;Olney,JW;Komisaruk,BR;Willis,WD;Bodnar,RJ
• 通讯作者: Bodnar,RJ

Immunohistochemical localization of corticotropin-releasing factor in selected brain areas of the European starling (Sturnus vulgaris) and the song sparrow (Melospiza melodia).

欧洲椋鸟 (Sturnus vulgaris) 和歌麻雀 (Melospiza melodia) 选定脑区促肾上腺皮质激素释放因子的免疫组织化学定位。

• DOI: 10.1007/bf00221646
• 发表时间: 1989
• 期刊: Cell and tissue research
• 影响因子: 3.6
• 作者: Ball,GF;Faris,PL;Wingfield,JC
• 通讯作者: Wingfield,JC