PRODUCTION OF REFERENCE REAGENTS FOR SAIDS AND STLV-III

SAIDS 和 STLV-III 参考试剂的生产

• 批准号: 3506001
• 负责人: DAVID A FUCCILLO
• 金额: $ 21.83万
• 依托单位: MICROBIOLOGICAL ASSOCIATES, INC.
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-04-01 至 1990-08-22
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3506001
• 关键词: AIDS; AIDS /HIV test; Pongidae; affinity labeling; agglutination reaction; antibody specificity; antigens; antiserum; antiviral antibody; avidin; biotin; diagnosis design /evaluation; disease /disorder model; enzyme linked immunosorbent assay; human immunodeficiency virus 1; human immunodeficiency virus 2; hybridomas; immunofluorescence technique; immunologic assay /test; laboratory mouse; monoclonal antibody; rapid diagnosis; reagent /indicator; serology /serodiagnosis; simian AIDSs; simian immunodeficiency virus; simian virus; tissue /cell culture; virus antigen; virus protein

Two distinct virally induced infections in non-human primates which cause substantial morbidity and mortality and with clinical relevance to human AIDS have been identified recently. The first, designated SAIDS, is caused by a variety of closely related Type D viruses (SRV-1, SRV-2). The second is induced by a lentivirus which is morphologically, biologically and serologically related to human HTLV-III (HIV-1) and HTLV-IV (HIV-2) and is designated STLV-III (SIV). These infections are of importance because they: (a) infect many non-human primates and may have major effects on experimentation in these species and (b) can serve as potential models for examining the biology, pathogenesis and potential therapy of AIDS-like diseases. Microbiological Associates, Inc. (MBA) proposes to produce monoclonal antibodies against a series of epitopes found on the simian retroviruses. These monoclonals along with polyclonal antibodies produced in Phase I studies will be used to develop simple, rapid antibody and antigen detection assays for SAIDS and SIV.

非人类灵长类动物中的两种不同的病毒感染 这会导致大量的发病率和死亡率以及临床 最近已经确定了与人类艾滋病的相关性。 这 首先，指定说是由各种密切相关的 D型病毒（SRV-1，SRV-2）。 第二个是由 慢病毒在形态，生物学上和血清学上是 与人类HTLV-III（HIV-1）和HTLV-IV（HIV-2）有关 指定的STLV-III（SIV）。 这些感染很重要 因为他们：（a）感染许多非人类灵长类动物，可能有 对这些物种的实验的主要影响，（b）可以 作为检查生物学的潜在模型，发病机理 和类似艾滋病疾病的潜在疗法。 Microbiological Associates，Inc。（MBA）提议生产 针对一系列表位的单克隆抗体在 猿猴逆转录病毒。 这些单克隆和多克隆 第一阶段研究产生的抗体将用于开发 简单，快速的抗体和抗原检测测定法和抗原检测分析 SIV。