PRODUCTION OF REFERENCE REAGENTS FOR SAIDS AND STLV-III

SAIDS 和 STLV-III 参考试剂的生产

• 批准号: 3488552
• 负责人: DAVID A FUCCILLO
• 金额: $ 4.99万
• 依托单位: MICROBIOLOGICAL ASSOCIATES, INC.
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-04-01 至 1987-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3488552
• 关键词: AIDS; RNA directed DNA polymerase; Retroviridae; antiserum; antiviral antibody; biological products; disease /disorder model; enzyme linked immunosorbent assay; human immunodeficiency virus 1; immunofluorescence technique; immunologic assay /test; immunological substance; reagent /indicator; serology /serodiagnosis; serotyping; simian virus; tissue /cell culture; virus classification; virus cytopathogenic effect; virus replication

Two distinct virally induced infections in non-human primates which cause substantial morbidity and mortality and with clinical relevance to human AIDS have been identified recently. The first, designated SAIDS, is caused by a variety of closely related Type D viruses. The second is induced by a lentavirus which is morphologically, biologically and serologically related to human HTLV-III and HTLV-IV and is designated STLV-III. These infections are of importance because they: (a) infect many non-human primates and may have major effects on experimentation in these species and (b) can serve as potential models for examining the biology, pathogenesis and potential therapy of AIDS-like diseases. Microbiological Associates, Inc. (MAI) proposes to grow isolates of the SAIDS (SRV-1; SRV-2) viruses in Raji cells and the STLV-III virus in HUT-78 cells. Standardized reagents (i.e. virally infected cells, both homologous antisera from infected monkeys and heterologous antisera from rabbits immunized with the whole virus isolates) would be characterized. These reagents would then be used to develop assays for the rapid identification of animals exposed to the virus.

非人类灵长类动物中的两种不同的病毒感染，引起 大量发病率和死亡率以及与人类的临床相关性 最近已经确定了艾滋病。 指定的说法是第一个是引起的 通过各种密切相关的D型病毒。 第二个是由 垒易塔病毒在形态学，生物学和血清学上相关 授予人类HTLV-III和HTLV-IV，并被指定为STLV-III。 这些感染 很重要，因为它们：（a）感染许多非人类灵长类动物，可能 对这些物种的实验有重大影响，（b）可以用作 研究生物学，发病机理和潜力的潜在模型 艾滋病样疾病的治疗。 Microbiological Associates，Inc。（MAI）提议种植分离株 在Raji细胞中说（SRV-1； SRV-2）病毒和HUT-78中的STLV-III病毒 细胞。 标准化试剂（即病毒感染的细胞，两个同源的细胞 来自受感染猴子的抗血清和兔子异源抗血清 用整个病毒分离株进行免疫）将被表征。 这些 然后，试剂将用于开发快速识别的测定 暴露于病毒的动物。