ENZYMES AS CATALYSTS IN THE SYNTHESIS OF CARBOHYDRATES

酶作为碳水化合物合成中的催化剂

• 批准号: 3484680
• 负责人: GEORGE M WHITESIDES
• 金额: $ 48.25万
• 依托单位: HARVARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-04-01 至 1995-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3484680
• 关键词: NAD(H) phosphate; adenosine triphosphate; carbohydrate analog; carbohydrate biosynthesis; carbohydrates; catalyst; chemical conjugate; chemical synthesis; cytosine nucleotides; enzyme induction /repression; enzyme inhibitors; enzyme mechanism; enzyme substrate; enzymes; esterase; gene expression; genetic manipulation; glycolipids; glycolysis; glycoproteins; glycosidases; glycosyltransferase; guanosine triphosphate; molecular cloning; monosaccharides; nicotinamide; nicotinamide adenine dinucleotide; nucleic acid chemical synthesis; oligosaccharides; organic chemicals; oxidoreductase; peptide chemical synthesis; phosphoenolpyruvate; phosphorylases; phosphotransferases; polysaccharides; proteins; solvents; starch; sugar phosphates; transaldolase /transketolase; uridine triphosphate

This research will develop enzymes as catalysts for the synthesis of carbohydrates and derivatives. It will: . Use aldolases and related enzymes -- particularly fructose-1,6- diphosphate aldolase, fuculose-1-phosphate aldolase, rhamnulose-1-phosphate aldolase and transketolase -- to make monosaccharides. . Synthesize the nucleoside phosphate sugars required for Leloir-pathway syntheses. . Continue to develop glycosyl transferases as catalysts for synthesis of oligosaccharides and polysaccharides via the Leloir pathway by practical procedures. . Use potato phosphorylase to prepare amylose conjugates of proteins and other molecules. . Develop strategies and techniques for using the products of enzyme- catalyzed syntheses of carbohydrates by preparing natural products and analogs of them: L-heptose, lincosamine, glycosidase inhibitors, neoglycoproteins, analogs of glycolipids. . Provide reagents and non-enzymatic reactions needed to support these efforts in enzyme-catalyzed synthesis.

这项研究将开发酶作为合成的催化剂 碳水化合物及其衍生物。 它将： 。使用醛缩酶和相关酶——特别是果糖-1,6- 二磷酸醛缩酶、1-磷酸岩藻糖醛缩酶、1-磷酸鼠李酮糖 醛缩酶和转酮醇酶——产生单糖。 。合成 Leloir 途径所需的核苷磷酸糖 综合。 。继续开发糖基转移酶作为合成的催化剂 寡糖和多糖通过 Leloir 途径通过实践 程序。 。使用马铃薯磷酸化酶制备蛋白质和直链淀粉缀合物 其他分子。 。制定使用酶产品的策略和技术 通过制备天然产物催化合成碳水化合物 它们的类似物：L-庚糖、林可胺、糖苷酶抑制剂、 新糖蛋白，糖脂的类似物。 。提供支持这些所需的试剂和非酶反应 酶催化合成方面的努力。