ENZYME CATALYZED SYNTHESIS OF MONOSACCHARIDES

酶催化单糖合成

• 批准号: 2684736
• 负责人: GEORGE M WHITESIDES
• 金额: $ 52.94万
• 依托单位: HARVARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2684736
• 关键词: NAD(H) phosphate; adenosine triphosphate; carbohydrate analog; carbohydrate biosynthesis; carbohydrates; catalyst; chemical conjugate; chemical synthesis; cytosine nucleotides; enzyme induction /repression; enzyme inhibitors; enzyme mechanism; enzyme substrate; enzymes; esterase; gene expression; genetic manipulation; glycolipids; glycolysis; glycoproteins; glycosidases; glycosyltransferase; guanosine triphosphate; molecular cloning; monosaccharides; nicotinamide; nicotinamide adenine dinucleotide; nucleic acid chemical synthesis; nucleotides; oligosaccharides; organic chemicals; oxidoreductase; peptide chemical synthesis; phosphoenolpyruvate; phosphorylases; phosphotransferases; polysaccharides; proteins; solvents; starch; sugar phosphates; transaldolase /transketolase; uridine triphosphate

This research will develop enzymes as catalysts for the synthesis of carbohydrates and derivatives. It will: . Use aldolases and related enzymes -- particularly fructose-1,6- diphosphate aldolase, fuculose-1-phosphate aldolase, rhamnulose-1-phosphate aldolase and transketolase -- to make monosaccharides. . Synthesize the nucleoside phosphate sugars required for Leloir-pathway syntheses. . Continue to develop glycosyl transferases as catalysts for synthesis of oligosaccharides and polysaccharides via the Leloir pathway by practical procedures. . Use potato phosphorylase to prepare amylose conjugates of proteins and other molecules. . Develop strategies and techniques for using the products of enzyme- catalyzed syntheses of carbohydrates by preparing natural products and analogs of them: L-heptose, lincosamine, glycosidase inhibitors, neoglycoproteins, analogs of glycolipids. . Provide reagents and non-enzymatic reactions needed to support these efforts in enzyme-catalyzed synthesis.

这项研究将开发酶作为合成的催化剂 碳水化合物和衍生物。 它将： 。使用藻酶和相关酶 - 尤其是果糖1,6-- 二磷酸醛酸酶，岩藻糖1-磷酸醛酸酶，鼻nulose-1-磷酸盐 藻酶和转酮酶 - 生成单糖。 。合成Leloir-Pathway所需的核苷磷酸糖 合成。 。继续开发糖基转移酶作为合成的催化剂 通过Leloir途径通过实用的寡糖和多糖 程序。 。使用马铃薯磷酸化酶制备蛋白质和 其他分子。 。制定使用酶产物的策略和技术 - 通过准备天然产物和 它们的类似物：l-甲糖，林糖胺，糖苷酶抑制剂， 新糖蛋白，糖脂的类似物。 。提供支持这些试剂和非酶反应 酶催化合成的努力。