CENTRAL CARDIOVASCULAR CONTROL: NORMAL AND HYPERTENSIVE

中央心血管控制：正常和高血压

• 批准号: 3449966
• 负责人: SHAUN F MORRISON
• 金额: $ 5.32万
• 依托单位: WEILL MEDICAL COLL OF CORNELL UNIV
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-07-01 至 1988-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3449966
• 关键词: antidromic impulse; baroreceptors; blood pressure; electrophysiology; gamma aminobutyrate; histochemistry /cytochemistry; hypertension; immunochemistry; neural information processing; neurotransmitters; nontherapeutic iontophoresis; reflex; sympathetic nervous system; synapses

Recent research on central cardiovascular control indicates the existence of a population of neurons in the lateral medullary reticular formation that provides a final common pathway conveying the excitatory drive to the spinal sympathetic nucleus that is critical for the maintenance of basal sympathetic tone. As described in the proposal, the characterization of these neurons will provide new information on the central neural mechanisms underlying the sympathetic control of blood pressure. The proposed studies will focus on the hypothesis that neurons in the rostral ventrolateral medulla (RVL) that contain the adrenaline-synthesizing enzyme, phenylethanolamine N-methyltransferase (PNMT) mediate the tonic bulbospinal excitation of sympathetic preganglionic neurons (SPN) necessary for the support of resting blood pressure. Extracellular recording and antidromic activation techniques are combined with baroreceptor reflex activation and computer-aided analysis to identify sympathoexcitatory neurons in RVL that project to the sympathetic intermediolateral nucleus. Immunocytochemical staining is used to determine the proximity of the recording sites of these neurons to the locations of neurons containing PNMT (C1 cell group). Additional experiments are designed to examine (1) the baroreceptor reflex pathway providing an inhibitory control of the activity of these cells and (2) the activation of RVL sympathetic neurons from hypothalamic pressor sites. Microiontophoretic application of antagonist drugs will be used to classify the transmitter system(s) mediating the effects of RVL activation on the activity of SPNs. Together these data would not only increase our knowledge of the basic neural mechanisms involved in central blood pressure regulation, they might also provide a basis for (1) the evaluation of the role of altered neural function in hypertensive disease and (2) the design of pharmacologic approaches to antihypertensive therapy. The former possibility is tested in a final series of experiments to determine if the sympathetic hyperactivity of the spontaneously hypertensive rat is associated with increased activity of the sympathoexcitatory neurons in the C1 region of the RVL.

关于中央心血管控制的最新研究表明存在 外侧网状形成中神经元的种群 这提供了最终的公共途径，将兴奋性驱动器传达给 脊柱交感核对于维持基础至关重要 同情的语气。 如提议中所述，表征 这些神经元将提供有关中央神经机制的新信息 血压的交感神经控制。 提出的研究 将重点介绍以下假说，即神经元的腹侧神经元 含有肾上腺素合成酶的髓质（RVL）， 苯甲胺N-甲基转移酶（PNMT）介导强调球囊 激发同情性神经神经元（SPN）的激发 支持静止的血压。 细胞外记录和抗原 激活技术与压力感受器反射激活相结合，并且 计算机辅助分析以识别RVL中的交感神经兴奋性神经元 投影到交感神经中间核。 免疫细胞化学 染色用于确定这些记录位点的接近性 神经元到包含PNMT的神经元位置（C1细胞组）。 其他实验旨在检查（1）压力感受器反射 提供对这些细胞活性的抑制控制和 （2）下丘脑压力机的RVL交感神经元激活 站点。 拮抗剂药物的微观遗迹应用将用于 对介导RVL激活的影响的发射机系统进行分类 关于SPN的活动。 这些数据不仅会增加我们的 了解中央血压涉及的基本神经机制 法规，他们还可能为（1）评估的基础 神经功能改变在高血压疾病中的作用，（2）设计 降压治疗的药理学方法。 前者 在最后一系列实验中测试了可能性，以确定是否是否 自发性高血压大鼠的交感神经多动症为 与交感神经兴奋性神经元的活性增加有关 RVL的C1区域。